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Bioactive grade

Native human Thrombin protein (Active) (ab62452)

Submit a review Q&A (2)References (4)
SDS-PAGE - Native human Thrombin protein (Active) (ab62452)

    Key features and details

    • Expression system: Native
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Suitable for: SDS-PAGE, Functional Studies

    製品の詳細

    • 製品名

      Native human Thrombin protein (Active)
      Thrombin タンパク質・ペプチド 製品一覧

    • 生理活性

      This product can be used for cleavage of fusion proteins. The activity varies form lot to lot. It is typically 3800 U/mg.

    • 精製度

      > 95 % SDS-PAGE.

    • 発現系

      Native

    • アクセッション番号

      P00734

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Native

      • 生物種

        Human

    特性

    Our Abpromise guarantee covers the use of ab62452 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

      Functional Studies

    • 製品の状態

      Liquid

    • 備考

      Concentration varies from lot to lot but is between 5mg/ml and 10mg/ml.

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

      Constituent: 50% Glycerol

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    関連情報

    • 別名

      • Coagulation factor II
      • Coagulation factor II thrombin
      • EC 3.4.21.5
      • F2
      • Factor II
      • Prepro coagulation factor II
      • Prothrombin
      • Prothrombin B chain
      • PT
      • RPRGL2
      • Serine protease
      • THPH1
      • THRB_HUMAN
      • Thrombin heavy chain
      see all

    • 機能

      Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

    • 組織特異性

      Expressed by the liver and secreted in plasma.

    • 関連疾患

      Factor II deficiency
      Ischemic stroke
      Thrombophilia due to thrombin defect
      Pregnancy loss, recurrent, 2

    • 配列類似性

      Belongs to the peptidase S1 family.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 2 kringle domains.
      Contains 1 peptidase S1 domain.

    • 翻訳後修飾

      The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
      N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).

    • 細胞内局在

      Secreted, extracellular space.
    • Information by UniProt

    画像

    • SDS-PAGE - Native human Thrombin protein (Active) (ab62452)
      SDS-PAGE - Native human Thrombin protein (Active) (ab62452)
      SDS-PAGE analysis of ab62452 for 1ug protein.
      Novex 4-12% Bis-Tris Gel
      NR= non-reduced; R = reduced

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (4)

    ab62452 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab62452 は 4 報の論文で使用されています。

    • Oliveira-Silva R  et al. Single-Particle Plasmon Sensor to Monitor Proteolytic Activity in Real Time. ACS Appl Opt Mater 1:1661-1669 (2023). PubMed: 37915971
    • Schmidt C  et al. A multiparametric fluorescence assay for screening aptamer-protein interactions based on microbeads. Sci Rep 12:2961 (2022). PubMed: 35194086
    • Amick KA  et al. Plasma glycocholic acid and linoleic acid identified as potential mediators of mitochondrial bioenergetics in Alzheimer's dementia. Front Aging Neurosci 14:954090 (2022). PubMed: 36212044
    • Park HJ  et al. One-pot colorimetric detection of molecules based on proximity proteolysis reaction. Biosens Bioelectron 188:113349 (2021). PubMed: 34030090

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    ab62452
    Active full length protein
    How is the protein prepared? Is this native purified protein?
    Cleaved protein is active which would not be full length.

    Read More
    Answer


    I can confirm this is native fully active protein. The Prothrombin is purified directly from plasma. The Prothrombin is then activated to Thrombin via a Prothrombinase reaction.

    PROTEOLYTIC ACTIVATION OF PROTHROMBIN The serine protease α-thrombin is produced by proteolytic activation of the zymogen, prothrombin. The enzyme complex, prothrombinase, catalyzes the proteolysis of two peptide bonds in prothrombin, which gives rise to an NH2-terminal derived F1.2 region and the heterodimer, alpha-thrombin. Alpha-thrombin is composed of an A chain (Mr=6000) which is covalently linked to a B chain (Mr=31,000) through a single disulfide bond.

    I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Question

    Is the Thrombin protein ab62452 in it's active form?
    Do you know the unit amount supplied within the 100ug included?

    Read More
    Answer


    I have checked with the lab and this protein is indeed active.

    For lot GR147433 currently in stock, we have determined the activity to be 3034 U/mg therefore 303.4 U per 100 ug.

    Read More

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