Native human Gamma Thrombin protein (ab81592)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Native human Gamma Thrombin protein -
生理活性
<1% Thrombin activity. Specific activity is determined by Fibrinogen clotting assay relative to NIH standard thrombin. 1 NIH Unit = 1.15 IS Units -
精製度
> 95 % SDS-PAGE. -
発現系
Native -
タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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生物種
Human -
領域
328 to 622
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特性
Our Abpromise guarantee covers the use of ab81592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 6.50
Constituents: 0.1% Polyethylene glycol, 1.64% Sodium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Activation peptide fragment 1
- Activation peptide fragment 2
- Cf 2
see all -
機能
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Factor II deficiency
Ischemic stroke
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2 -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). -
細胞内局在
Secreted, extracellular space. - Information by UniProt
画像
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SDS-PAGE - Native human Gamma Thrombin protein (ab81592)Novex 4-12% Bis-Tris
1 µg per lane
MOPS
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab81592 は論文での使用が確認できていません。