AB62549

Native Human Factor X protein

(1 Review)

(2 Publications)

Native Human Factor X protein is a Human Full Length protein, expressed in Native, with >95%, suitable for SDS-PAGE, FuncS.

別名を表示する

Coagulation factor X, Stuart factor, Stuart-Prower factor, F10

1 Images

SDS-PAGE - Native Human Factor X protein (AB62549)

SDS-PAGE

Supplier Data

SDS-PAGE - Native Human Factor X protein (AB62549)

SDS-PAGE analysis of ab62549 for 1ug protein.
Novex 4-12% Bis-Tris Gel
NR= non-reduced; R = reduced

Key facts

精製度

>95% SDS-PAGE

発現系

Native

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

アクセッション番号

P00742

アニマルフリー

キャリアフリー

種

Human

バッファー組成

Constituents: 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Native","expressionSystem":null,"accessionNumber":"P00742","tags":[]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Factor X also known as Stuart-Prower Factor is a critical component in the coagulation cascade. It has a molecular weight of approximately 59 kDa and is expressed mainly in the liver. This serine protease plays an important role in the conversion of prothrombin to thrombin an essential step in blood clot formation. Factor X circulates in the plasma as an inactive zymogen and becomes activated to its enzyme form factor Xa in response to specific physiological signals.

Biological function summary

Factor X contributes significantly to the coagulation process by activating prothrombin into thrombin. It is not part of a larger complex but functions closely with other components of the coagulation cascade to maintain hemostasis. Factor X interacts with calcium ions and phospholipids on the surface of platelets enhancing its enzymatic activity. This activation ensures proper regulation of blood clotting preventing excessive bleeding or uncontrolled clot formation.

Pathways

Factor X is central to both the intrinsic and extrinsic coagulation pathways. It interacts with proteins such as factor VIIa in the extrinsic pathway and factor IXa in the intrinsic pathway both pathways leading to the activation of factor Xa. In turn factor Xa is key in the common pathway which combines inputs from the intrinsic and extrinsic pathways to produce thrombin essential for fibrin clot formation.

Factor X is associated with bleeding disorders such as hemophilia and liver disease. Deficiencies or dysfunctions in factor X result in inadequate clot formation leading to prolonged bleeding episodes. Furthermore increased factor X activity as in cases of thrombophilia can raise the risk of abnormal blood clots. Factor X works alongside proteins like factor V with both deficiencies playing roles in hemophilia. Monitoring factor X activity is important in diagnosing and managing these coagulation-related conditions.

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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed : 22409427). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed : 24041930, PubMed : 30568593, PubMed : 34831181, PubMed : 18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed : 35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed : 30568593, PubMed : 34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed : 18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed : 22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed : 24041930).

配列の類似性

Belongs to the peptidase S1 family.

翻訳後修飾

The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.. Proteolytically cleaved and activated by cathepsin CTSG (PubMed:8920993). The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway) (By similarity).. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information F10

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Acta biomaterialia 137:331-345 PubMed34673227

2021

Reduced thrombogenicity of surface-treated Nitinol implants steered by altered protein adsorption.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Gegenschatz-Schmid,Stefano Buzzi,Jonas Grossmann,Bernd Roschitzki,Riccardo Urbanet,Roman Heuberger,Dorothea Glück,Arik Zucker,Martin Ehrbar

FASEB journal : official publication of the Federa 33:3954-3967 PubMed30517034

2018

Therapeutic expression of human clotting factors IX and X following adeno-associated viral vector-mediated intrauterine gene transfer in early-gestation fetal macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Jerry K Y Chan,Irene Gil-Farina,Nuryanti Johana,Cecilia Rosales,Yi Wan Tan,Jessika Ceiler,Jenny Mcintosh,Bryan Ogden,Simon N Waddington,Manfred Schmidt,Arijit Biswas,Mahesh Choolani,Amit C Nathwani,Citra N Z Mattar

View all publications

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Native Human Factor X protein