Native Human Collagen VI protein (ab7538)
Key features and details
- Expression system: Native
- Suitable for: SDS-PAGE, WB, IP, ELISA, IHC-P
製品の詳細
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製品名
Native Human Collagen VI protein
Collagen VI タンパク質・ペプチド 製品一覧 -
発現系
Native -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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生物種
Human -
配列の追加情報
Prepared from Human Placenta.
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特性
Our Abpromise guarantee covers the use of ab7538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
Immunoprecipitation
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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製品の状態
Liquid -
備考
This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type VI. Reaction with anti-Collagen I, II, III, IV or V is negligible (typically less than 1% cross reactivity was detected by ELISA).
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C. Diluted stock solutions should be used immediately and not be frozen. Please see notes section. Store undiluted.
Preservative: 0.01% Sodium azide
Constituent: 3% Acetic acid
関連情報
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別名
- Alpha 1 (VI) chain (61 AA)
- CO6A1_HUMAN
- COL6A1
see all -
機能
Collagen VI acts as a cell-binding protein. -
関連疾患
Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. -
配列類似性
Belongs to the type VI collagen family.
Contains 3 VWFA domains. -
翻訳後修飾
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab7538 は 5 報の論文で使用されています。
- Papalazarou V et al. Collagen VI expression is negatively mechanosensitive in pancreatic cancer cells and supports the metastatic niche. J Cell Sci 135:N/A (2022). PubMed: 36546396
- Holm Nielsen S et al. A Fragment of Collagen Type VI alpha-3 chain is Elevated in Serum from Patients with Gastrointestinal Disorders. Sci Rep 10:5910 (2020). PubMed: 32245981
- Wishart AL et al. Decellularized extracellular matrix scaffolds identify full-length collagen VI as a driver of breast cancer cell invasion in obesity and metastasis. Sci Adv 6:N/A (2020). PubMed: 33087348
- Spiers RM et al. Donor age significantly influences the Raman spectroscopic biomolecular fingerprint of human pancreatic extracellular matrix proteins following collagenase-based digestion. Acta Biomater 99:269-283 (2019). PubMed: 31525537
- Veidal SS et al. MMP Mediated Degradation of Type VI Collagen Is Highly Associated with Liver Fibrosis - Identification and Validation of a Novel Biochemical Marker Assay. PLoS One 6:e24753 (2011). PubMed: 21935455