Native Human Collagen IV protein (ab7536)
Key features and details
- Expression system: Native
- Suitable for: SDS-PAGE, WB, IP, ELISA, Blocking
製品の詳細
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製品名
Native Human Collagen IV protein
Collagen IV タンパク質・ペプチド 製品一覧 -
発現系
Native -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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生物種
Human -
予測される分子量
161 kDa -
配列の追加情報
Prepared from human placenta and is chromatographically and immunologically pure.
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特性
Our Abpromise guarantee covers the use of ab7536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
Immunoprecipitation
ELISA
Blocking
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製品の状態
Liquid -
備考
This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type IV. Reaction with anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Store undiluted.
Preservative: 0.01% Sodium azide
Constituent: 3% Acetic acid
関連情報
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別名
- Arresten
- BSVD
- CO4A1_HUMAN
see all -
機能
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. -
組織特異性
Highly expressed in placenta. -
関連疾患
Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles. -
配列類似性
Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain. -
ドメイン
Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain. -
翻訳後修飾
Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Proteolytic processing produces the C-terminal NC1 peptide, arresten. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab7536 は 12 報の論文で使用されています。
- Fatherree JP et al. Chemotherapy-Induced Collagen IV Drives Cancer Cell Motility through Activation of Src and Focal Adhesion Kinase. Cancer Res 82:2031-2044 (2022). PubMed: 35260882
- Jk L et al. Lack of autoantibodies against collagen and related proteins in collagenous colitis. BMC Immunol 23:29 (2022). PubMed: 35668375
- Zambuto SG et al. Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels. ACS Biomater Sci Eng 8:3819-3830 (2022). PubMed: 35994527
- Wang S et al. MiR-1297 attenuates high glucose-induced injury in HK-2 cells via targeting COL1A2. Nephrology (Carlton) 26:623-631 (2021). PubMed: 33811432
- Yun J et al. Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy. Diabetol Metab Syndr 13:72 (2021). PubMed: 34174955