Mouse Lamin B1 peptide (ab16375)
Key features and details
- Purity: > 70% HPLC
- Suitable for: Blocking
製品の詳細
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製品名
Mouse Lamin B1 peptide -
精製度
> 70 % HPLC.
70 - 90% by HPLC -
アクセッション番号
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Animal free
No -
由来
Synthetic -
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生物種
Mouse
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関連製品
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Corresponding Antibody
特性
Our Abpromise guarantee covers the use of ab16375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Blocking - Blocking peptide for Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
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製品の状態
Lyophilized -
備考
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Information available upon request.
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再構成Reconstitute with 100 ul of deionised water (or equivalent).
関連情報
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別名
- ADLD
- lamin B1
- Lamin-B1
see all -
機能
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
関連疾患
Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
細胞内局在
Nucleus inner membrane. - Information by UniProt
画像
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Western blot - Mouse Lamin B1 peptide (ab16375)All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate withMouse Lamin B1 peptide (ab16375) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa fluor goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab16375 は 3 報の論文で使用されています。
- Ahanger SH et al. Distinct nuclear compartment-associated genome architecture in the developing mammalian brain. Nat Neurosci 24:1235-1242 (2021). PubMed: 34239128
- Luzhin AV et al. Chromatin Trapping of Factors Involved in DNA Replication and Repair Underlies Heat-Induced Radio- and Chemosensitization. Cells 9:N/A (2020). PubMed: 32521766
- Roig MB et al. Identification of a novel cyclin required for the intrinsic apoptosis pathway in lymphoid cells. Cell Death Differ : (2008). PubMed: 18927588
