Human IgE protein (Azide free) (ab65866)
Key features and details
- Expression system: Monoclonal Hybridoma
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 25.000 Eu/mg
- Active: Yes
- Suitable for: SDS-PAGE, IHC - Wholemount, WB, ELISA, ICC
製品の詳細
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製品名
Human IgE protein (Azide free)
IgE タンパク質・ペプチド 製品一覧 -
生理活性
1 mg of ab65866 is 380,000± 50,000 IU/ml.
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精製度
> 90 % SDS-PAGE.
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum. -
エンドトキシン・レベル
< 25.000 Eu/mg -
発現系
Monoclonal Hybridoma -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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アミノ酸配列 1
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生物種
Human -
配列の追加情報
Observed band of ~185kDa on SDS-PAGE
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アミノ酸配列 2
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生物種
Human
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特性
Our Abpromise guarantee covers the use of ab65866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
IHC - Wholemount
Western blot
ELISA
Immunocytochemistry
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製品の状態
Liquid -
備考
This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.
This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.
- Low batch-to-batch variation
- Higher degree of purity (>90%)
- A more uniform product (monoclonal and not polyclonal)
Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cell proliferation studies.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.00017% PBS, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Igh2
- IGHE
- IGHEP1
- Immunoglobulin heavy constant epsilon
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関連性
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
細胞内局在
Cell Membrane
画像
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Sandwich ELISA - Native Human IgE protein (Azide free) (ab65866)
Background-subtracted data values (mean +/- SD) are graphed.
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SDS-PAGE - Native Human IgE protein (Azide free) (ab65866)SDS-PAGE analysis of:
Lane 1: Molecular weight markers.
Lane 2: ab65866.
Lane 3: IgE purified from serum from a myeloma patient.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (10)
ab65866 は 10 報の論文で使用されています。
- Lindenburg L et al. Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins. Nucleic Acids Res 48:e63 (2020). PubMed: 32383757
- Lafleur MA et al. MRGPRX2 activation as a rapid, high-throughput mechanistic-based approach for detecting peptide-mediated human mast cell degranulation liabilities. J Immunotoxicol 17:110-121 (2020). PubMed: 32525431
- Hotta F et al. Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear. Int J Mol Med 46:1367-1376 (2020). PubMed: 32945368
- Vidal M et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). PubMed: 29397157
- Wan Z et al. Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing. PLoS One 13:e0191987 (2018). PubMed: 29389948
- Khramtsov P et al. Highly Stable Conjugates of Carbon Nanoparticles with DNA Aptamers. Langmuir N/A:N/A (2018). PubMed: 30089209
- Cui L et al. JSI-124 inhibits IgE production in an IgE B cell line. Biochem Biophys Res Commun 483:669-673 (2017). PubMed: 27988336
- van Beek N et al. Correlation of Serum Levels of IgE Autoantibodies Against BP180 With Bullous Pemphigoid Disease Activity. JAMA Dermatol N/A:N/A (2016). PubMed: 27829102
- Baltabekova AZh et al. SplitCore Technology Allows Efficient Production of Virus-Like Particles Presenting a Receptor-Contacting Epitope of Human IgE. Mol Biotechnol 57:746-55 (2015). PubMed: 25837568
- Goodman RE et al. Evaluation of endogenous allergens for the safety evaluation of genetically engineered food crops: review of potential risks, test methods, examples and relevance. J Agric Food Chem 61:8317-32 (2013). SDS-PAGE . PubMed: 23848840
