AB65866

Native Human IgE protein (Azide free)

Name: Human IgE protein (Azide free) (ab65866) | Abcam
Brand: Abcam Limited
SKU: AB65866
Price: 74000 JPY
Availability: InStock
Rating: 4 (2 reviews)

Human IgE protein (Azide free)

(2 Reviews)

(12 Publications)

Human IgE protein (Azide free) is a Human Full Length IgE protein with >90% purity, < 25 EU/mg endotoxin level and suitable for western blot, ELISA, IHC, and SDS-PAGE. The predicted molecular weight of ab65866 protein is 185 kDa.

- Save time and ensure accurate results - use ab65866 as an IgE isotype control
- Optimal bioactivity, stability and reproducibility
- No contamination from other immunoglobulin isotypes
- Available in different sizes to fit your experimental needs

別名を表示する

Immunoglobulin epsilon heavy chain, Immunoglobulin epsilon heavy chain ND, Immunoglobulin kappa light chain, Immunoglobulin kappa light chain EU

2 Images

SDS-PAGE - Human IgE protein (Azide free) (AB65866)

SDS-PAGE

Supplier Data

SDS-PAGE - Human IgE protein (Azide free) (AB65866)

SDS-PAGE analysis of ab65866.
Lane 1 : Molecular weight markers.
Lane 2 : ab65866 at molecular weight 185 kDa.
Lane 3 : IgE purified from serum from a myeloma patient.

Sandwich ELISA - Human IgE protein (Azide free) (AB65866)

sELISA

Unknown

Sandwich ELISA - Human IgE protein (Azide free) (AB65866)

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

精製度

>90% SDS-PAGE

Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.

エンドトキシンレベル

< 25 EU/mg

発現系

Native

アプリケーション

ELISA, FuncS, WB, IHC, SDS-PAGE

applications

生物活性

Yes

生物学的活性

1 mg of ab65866 is 380,000± 50,000 IU/ml.

アクセッション番号

P0DOX4

アニマルフリー

キャリアフリー

Yes

種

Human

バッファー組成

pH: 7.4 Constituents: PBS, 0.88% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Observed band of ~185kDa on SDS-PAGE" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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製品の詳細

Ensure the validity of your result using our Immunoglobulin E (IgE) protein ab65866 as an isotype control. ab65866 can be used as a positive control in SDS-PAGE and western blot assays.
Analyze your ELISA data using the IgE ab65866 protein to generate and plot the standard curve.

Check out our ELISA protocol for more information here.

This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.

This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line.

Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients.

A comparison which is highly superior on the following key parameters:
- Low batch-to-batch variation
- Higher degree of purity (>90%)
- A more uniform product (monoclonal and not polyclonal)

Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cell proliferation studies.

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配列情報

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"","expressionSystem":null,"accessionNumber":"P0DOX4","tags":[]},{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"","expressionSystem":null,"accessionNumber":"P0DOX7","tags":[]}]

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出荷温度及び保存条件

製品の状態

Liquid

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The IgE protein [also known as immunoglobulin E] is a type of antibody with a molecular mass of approximately 190 kDa. IgE is expressed primarily on the surface of mast cells and basophils. It plays a central role in the immune system by binding to allergens and triggering the release of inflammatory mediators from mast cells and basophils. IgE's high-affinity binding to its receptor FcεRI on these effector cells facilitates this process. IgE's activity is central to initiating immediate hypersensitivity reactions which are a part of the body's immune response.

Biological function summary

The IgE protein functions as a mediator in allergic reactions. It does not operate as part of a complex but directly interacts with allergens and the IgE-specific receptors on immune cells. IgE's role is important in defense against parasitic infections where it performs a protective function by promoting eosinophil activation and degranulation. This action helps the body to expel the parasites. In the absence of parasitic infection IgE's function translates into its role in allergic responses where it becomes responsible for the symptoms associated with allergic diseases.

Pathways

IgE is an important player in the immune response pathway. The protein interacts with allergy-related pathways such as the T helper 2 (Th2) pathway. IgE's role in this pathway involves cooperation with cytokines notably IL-4 and IL-13 which are produced by Th2 cells and promote IgE synthesis in B cells. Also IgE is linked to the FcεRI receptor signaling pathway that initiates the release of histamines and other mediators when IgE binds to an allergen leading to an allergic response.

IgE is closely linked to allergic conditions like asthma and allergic rhinitis. These conditions result from IgE-mediated inflammatory responses that affect the respiratory system. In asthma IgE antibodies play a role in airway inflammation and hyperreactivity by promoting the activation of multiple immune cells including eosinophils and mast cells. IgE's involvement in allergic rhinitis manifests through similar mechanisms with nasal inflammation and irritation. IgE's interaction with other proteins such as histamine-releasing factor exacerbates these conditions by increasing the release of histamine which intensifies the allergic symptoms.

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一般的な情報

機能

Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Immunoglobulin epsilon heavy chain

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

BMC biotechnology 24:72 PubMed39367362

2024

Microarray-based evaluation of selected recombinant timothy grass allergens expressed in E. Coli and N. Benthamiana.

Applications

Unspecified application

Species

Unspecified reactive species

Laimis Silimavicius,Lieve Tchebotarev,Mindaugas Zaveckas,Raimundas Razanskas,Laima Cepulyte,Karolina Bielske,Indre Kucinskaite-Kodze,Linas Griguola,Kotryna Linauskiene,Rasa Petraityte-Burneikiene

The Journal of allergy and clinical immunology 152:711-724.e14 PubMed37100120

2023

Siglec-9 is an inhibitory receptor on human mast cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Miralda,Nyssa B Samanas,Albert J Seo,Jake S Foronda,Josie Sachen,Yvonne Hui,Shane D Morrison,Carole A Oskeritzian,Adrian M Piliponsky

International journal of molecular medicine 46:1367-1376 PubMed32945368

2020

Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear.

Applications

Unspecified application

Species

Unspecified reactive species

Fumika Hotta,Hiroshi Eguchi,Haruyuki Nakayama-Imaohji,Tomomi Kuwahara,Ayano Tada,Hirofumi Yagi,Yoshikazu Shimomura,Shunji Kusaka

Journal of immunotoxicology 17:110-121 PubMed32525431

2020

MRGPRX2 activation as a rapid, high-throughput mechanistic-based approach for detecting peptide-mediated human mast cell degranulation liabilities.

Applications

Unspecified application

Species

Unspecified reactive species

Marc A Lafleur,Jonathan Werner,Madeline Fort,Edward K Lobenhofer,Mercedesz Balazs,Ana Goyos

Nucleic acids research 48:e63 PubMed32383757

2020

Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Laurens Lindenburg,Tuomas Huovinen,Kayleigh van de Wiel,Michael Herger,Michael R Snaith,Florian Hollfelder

Langmuir : the ACS journal of surfaces and colloids 34:10321-10332 PubMed30089209

2018

Highly Stable Conjugates of Carbon Nanoparticles with DNA Aptamers.

Applications

Unspecified application

Species

Unspecified reactive species

Pavel Khramtsov,Maria Kropaneva,Tatyana Kalashnikova,Maria Bochkova,Valeria Timganova,Svetlana Zamorina,Mikhail Rayev

PloS one 13:e0191987 PubMed29389948

2018

Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Wan,Heather P Ostendorff,Ziying Liu,Lynda C Schneider,Kenneth J Rothschild,Mark J Lim

Journal of immunological methods 455:41-54 PubMed29397157

2018

Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Vidal,Ruth Aguilar,Joseph J Campo,Carlota Dobaño

Biochemical and biophysical research communications 483:669-673 PubMed27988336

2016

JSI-124 inhibits IgE production in an IgE B cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Cui,Jiacheng Bi,Dehong Yan,Xiufeng Ye,Mingxing Zheng,Guang Yu,Xiaochun Wan

JAMA dermatology 153:30-38 PubMed27829102

2016

Correlation of Serum Levels of IgE Autoantibodies Against BP180 With Bullous Pemphigoid Disease Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Nina van Beek,Nadine Lüttmann,Franziska Huebner,Andreas Recke,Ingolf Karl,Franziska Sophie Schulze,Detlef Zillikens,Enno Schmidt

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Native Human IgE protein (Azide free)

Human IgE protein (Azide free)

SDS-PAGE - Human IgE protein (Azide free) (AB65866)

Sandwich ELISA - Human IgE protein (Azide free) (AB65866)

Key facts

精製度

エンドトキシンレベル

発現系

タグ

アプリケーション

生物活性

生物学的活性

アクセッション番号

アニマルフリー

キャリアフリー

種

バッファー組成

組合せ製品

Reagents, Controls & Accessories

Reagents, Controls & Accessories

Secondary Antibodies

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity data

製品の詳細

配列情報

出荷温度及び保存条件

製品の状態

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

一般的な情報

機能

製品プロトコール

ターゲットの情報

文献 (12)

Abcam product promise