Human IgE protein (ab152001)
Key features and details
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 25.000 Eu/mg
- Suitable for: ELISA, ICC, SDS-PAGE
製品の詳細
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製品名
Human IgE protein
IgE タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum. -
エンドトキシン・レベル
< 25.000 Eu/mg -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Synthetic -
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生物種
Human
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製品の詳細
Native Human IgE protein
特性
Our Abpromise guarantee covers the use of ab152001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
ELISA
Immunocytochemistry
SDS-PAGE
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製品の状態
Liquid -
備考
ab152001 is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B. Must be handled as potentially infectious as all Human material -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.88% Sodium chloride
関連情報
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別名
- Igh2
- IGHE
- IGHEP1
- Immunoglobulin heavy constant epsilon
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関連性
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
細胞内局在
Cell Membrane
画像
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SDS-PAGE - Native Human IgE protein (ab152001)
SDS-PAGE analysis of ab152001.
Lane 1: Marker
Lane 2: purified Human IgE (ab152001), MW 185 kDa
Lane 3: Myeloma source IgE, purified from Human serum.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab152001 は 1 報の論文で使用されています。
- Wagemakers M et al. COX-2 Inhibition Combined with Radiation Reduces Orthotopic Glioma Outgrowth by Targeting the Tumor Vasculature. Transl Oncol 2:1-7 (2009). PubMed: 19252746