AB1773

Human Histone H3 (tri methyl K9) peptide

Name: Human Histone H3 (tri methyl K9) peptide (ab1773) | Abcam
Brand: Abcam Limited
SKU: AB1773
Price: 82000 JPY
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(18 Publications)

Human Histone H3 (tri methyl K9) peptide is a Synthetic blocking peptide. >90% and suitable for BL.

別名を表示する

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9me3, H3K9me, H3K9

19 Images

Unknown

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

ab5819 is specific for Histone H3 phosphorylated at residue S10 and tri methylated at residue K9. The activity of the antibody is blocked by the addition of the immunizing peptide, ab15644 (lanes 3 and 4).

All lanes:

Western blot - Anti-Histone H3 (tri methyl K9, phospho S10) antibody (<a href='/products/primary-antibodies/histone-h3-tri-methyl-k9-phospho-s10-antibody-ab5819'>ab5819</a>) at 1/500 dilution

Lane 1:

HeLa histones at 0.5 µg

Lane 2:

Colcemid-treated HeLa histones at 0.5 µg

Lane 3:

HeLa histones at 0.5 µg with Human Histone H3 (tri methyl K9, phospho S10) peptide (ab15644)

Lane 4:

Colcemid-treated HeLa histones at 0.5 µg with Human Histone H3 (tri methyl K9, phospho S10) peptide (ab15644)

Lane 5:

HeLa histones at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 6:

Colcemid-treated HeLa histones at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 7:

HeLa histones at 0.5 µg with Human Histone H3 (phospho S10) peptide (<a href='/products/proteins-peptides/human-histone-h3-phospho-s10-peptide-ab11477'>ab11477</a>)

Lane 8:

Colcemid-treated HeLa histones at 0.5 µg with Human Histone H3 (phospho S10) peptide (<a href='/products/proteins-peptides/human-histone-h3-phospho-s10-peptide-ab11477'>ab11477</a>)

Lane 9:

HeLa histones at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 10:

Colcemid-treated HeLa histones at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 22 kDa,60 kDa

false

ChIP - Human Histone H3 (tri methyl K9) peptide (AB1773)

ChIP

Project

ChIP - Human Histone H3 (tri methyl K9) peptide (AB1773)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab78513 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Project

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

All lanes:

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-di-methyl-k36-antibody-chip-grade-ab9049'>ab9049</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide (ab41430)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K36) peptide (ab1783)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K36) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k36-peptide-ab1784'>ab1784</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K36) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k36-peptide-ab1785'>ab1785</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k27-peptide-ab1782'>ab1782</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Project

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

ab78513 is blocked by the addition of Histone H4 (mono methyl K20)peptide (ab17043; lane 6). ab78513 is partially blocked by Histone H4 (di methyl K20) peptide (lane 7).

All lanes:

Western blot - Anti-Histone H4 (mono methyl K20) antibody [5E10-D8] - ChIP Grade (<a href='/products/primary-antibodies/histone-h4-mono-methyl-k20-antibody-5e10-d8-chip-grade-ab78513'>ab78513</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H4 peptide (ab14963)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H4 (mono methyl K20) peptide (ab17043)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Histone H4 peptide (17-24) - di methyl K20

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H4 (tri methyl K20) peptide (<a href='/products/proteins-peptides/human-histone-h4-tri-methyl-k20-peptide-ab17567'>ab17567</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa,17 kDa

true

Exposure time: 1min

Unknown

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

The peptide competition assay shows that this antibody is very specific for Histone H3 di-methylated at K27. It does not recognise Histone H3 mono, di, or tri methylated at K4, Histone H3 mono, di, or tri methylated at K9, or Histone H3 mono, or tri methylated at K27.

All lanes:

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-di-methyl-k27-antibody-chip-grade-ab24684'>ab24684</a>) at 1 µg/mL

Lane 1:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Human Histone H3 peptide (ab17163)

Lane 3:

Calf thymus histone lysate with Human Histone H3 (mono methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf thymus histone lysate with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf thymus histone lysate with Human Histone H3 (tri methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf thymus histone lysate with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 9:

Calf thymus histone lysate with Human Histone H3 (mono methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf thymus histone lysate with Human Histone H3 (di methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf thymus histone lysate with Human Histone H3 (tri methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k27-peptide-ab1782'>ab1782</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG H&L (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 5s

Lab

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

ab78517 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 8 where the activity of ab78517 is specifically blocked by the addition of the immunizing peptide (ab17567).

All lanes:

Western blot - Anti-Histone H4 (di methyl K20, tri methyl K20) antibody [6F8-D9] (<a href='/products/primary-antibodies/histone-h4-di-methyl-k20-tri-methyl-k20-antibody-6f8-d9-ab78517'>ab78517</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 peptide (ab14963)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 (mono methyl K20) peptide (ab17043)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H4 peptide (17-24) - di methyl K20

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 (tri methyl K20) peptide (<a href='/products/proteins-peptides/human-histone-h4-tri-methyl-k20-peptide-ab17567'>ab17567</a>)

Lane 9:

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

true

Exposure time: 90s

Project

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

All lanes:

Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-mono-methyl-k9-antibody-chip-grade-ab9045'>ab9045</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Lane 4:

Lane 5:

Lane 6:

Lane 7:

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k27-peptide-ab1782'>ab1782</a>)

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 15 kDa

false

Project

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

ab8896 specifically recognises mono-methyl K9 (lane 1) in calf thymus lysate at 17kDa. ab8896 is successfully blocked using the immunizing peptide (lane 2 ab1771), but not the di-methyl K9 (lane 3 ab1772), the tri-methyl K9 (lane 4 ab1773), the mono-methyl K27 (lane 5 ab1780), the di-methyl K27 (lane 6 ab1781), the tri-methyl K27 (lane 7 ab1782), the mono-methyl K4 (lane 8 ab1340) nor the corresponding unmodified peptides (lane 9 ab7228, lane 10 ab2623). This implies that ab8896 is specific to mono-methyl K9.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-mono-methyl-k9-antibody-chip-grade-ab8896'>ab8896</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 3:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 4:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 5:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 6:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 7:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k27-peptide-ab1782'>ab1782</a>)

Lane 8:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 9:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 10:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2623'>ab2623</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Project

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

All lanes : Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

Lane 1 : Calf thymus histone lysate
Lane 2 : Calf thymus histone lysate with Histone H3 peptide - unmodified at 1 μg/ml
Lane 3 : Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (ab1771) at 1 μg/ml
Lane 4 : Calf thymus histone lysate with Human Histone H3 (di methyl K9) peptide (ab1772) at 1 μg/ml
Lane 5 : Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (ab1773) at 1 μg/ml
Lane 6 : Calf thymus histone lysate with Histone H3 (di methyl K4) peptide (ab7768) at 1 μg/ml
Lane 7 : Calf thymus histone lysate with Human Histone H3 (di methyl K27) peptide (ab1781) at 1 μg/ml

Secondary
Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 17 kDa

Exposure time : 1 minute

All lanes:

Western blot - Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-di-methyl-k9-antibody-mabcam-1220-chip-grade-ab1220'>ab1220</a>) at 1 µg/mL

Lane 1:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Histone H3 peptide - unmodified

Lane 3:

Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 4:

Calf thymus histone lysate with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 5:

Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 6:

Calf thymus histone lysate with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 7:

Calf thymus histone lysate with Human Histone H3 (di methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (<a href='/products/secondary-antibodies/rabbit-mouse-igg-h-l-hrp-ab6728'>ab6728</a>) at 1/5000 dilution

Predicted band size: 15 kDa

true

Exposure time: 1min

Lab

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

Lane 8 shows that Rabbit polyclonal to Histone H3 (tri methyl K9) is blocked by the addition of the immunizing peptide (ab1773). Cross-reactivity with Histone H3 peptide - tri methyl K27 (ab1782) is also shown in Lane 11.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-tri-methyl-k9-antibody-chip-grade-ab8898'>ab8898</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 9:

Lane 10:

Lane 11:

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

All lanes:

Western blot - HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (<a href='/products/primary-antibodies/hrp-histone-h3-di-methyl-k9-antibody-mabcam-1220-ab62168'>ab62168</a>) at 1/20000 dilution

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2903'>ab2903</a>)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 9:

Lane 10:

Lane 11:

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Lab

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6147 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (<a href='/products/primary-antibodies/histone-h3-di-methyl-k27-tri-methyl-k27-antibody-mabcam-6147-ab6147'>ab6147</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2623'>ab2623</a>)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 9:

Lane 10:

Lane 11:

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 1min

Lab

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab78517).

ab78517 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 8 where the activity of ab78517 is specifically blocked by the addition of the immunizing peptide (ab17567).

All lanes:

Western blot - Anti-Histone H4 (di methyl K20, tri methyl K20) antibody [6F8-D9] - BSA and Azide free (<a href='/products/primary-antibodies/histone-h4-di-methyl-k20-tri-methyl-k20-antibody-6f8-d9-bsa-and-azide-free-ab264528'>ab264528</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 peptide (ab14963)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 (mono methyl K20) peptide (ab17043)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H4 peptide (17-24) - di methyl K20

Lane 9:

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

true

Exposure time: 90s

Project

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab12209).

All lanes:

Western blot - Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-tri-methyl-k4-antibody-mabcam12209-chip-grade-ab12209'>ab12209</a>) at 2 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 9:

Lane 10:

Lane 11:

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 16min

Project

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

All lanes:

Western blot - Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h4-mono-methyl-k20-antibody-chip-grade-ab9051'>ab9051</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H4 peptide (ab14963)

Lane 3:

Lane 4:

Lane 5:

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H4 (mono methyl K20) peptide (ab17043)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Histone H4 peptide (17-24) - di methyl K20

Lane 8:

Lane 9:

Lane 10:

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

false

Exposure time: 10s

Project

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

ab9053 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 5 where the activity of ab9053 is specifically blocked by the addition of the immunizing peptide (ab17567).

All lanes:

Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h4-tri-methyl-k20-antibody-chip-grade-ab9053'>ab9053</a>) at 1 µg/mL

Lane 1:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Human Histone H4 peptide (ab2622)

Lane 3:

Calf thymus histone lysate with Human Histone H4 (mono methyl K20) peptide (ab17043)

Lane 4:

Calf thymus histone lysate with Human Histone H4 (di methyl K20) peptide (ab14964)

Lane 5:

Calf thymus histone lysate with Human Histone H4 (tri methyl K20) peptide (<a href='/products/proteins-peptides/human-histone-h4-tri-methyl-k20-peptide-ab17567'>ab17567</a>)

Lane 6:

Calf thymus histone lysate with Human Histone H3 (tri methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 7:

Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (ab1773)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

false

Project

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

Expected molecular weights : H3 = 17 kDa; H4 = 14 kDa This image shows that the main epitopes recognized by ab7315 are the di methylated lysine residues. This can be seen in lanes 4, 7 and 10 where the activity of the antibody is quenched by the immunizing peptides (ab7768, ab1772, ab1781).

All lanes:

Western blot - Anti-pan methyl Lysine antibody - ChIP Grade (<a href='/products/primary-antibodies/pan-methyl-lysine-antibody-chip-grade-ab7315'>ab7315</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 9:

Lane 10:

Lane 11:

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 14-17 kDa

true

Exposure time: 20min

Unknown

Western blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

All lanes:

Western blot - Anti-Histone H3 (di methyl K4, tri methyl K4) antibody [mAbcam 6000] - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-di-methyl-k4-tri-methyl-k4-antibody-mabcam-6000-chip-grade-ab6000'>ab6000</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (ab1773)

Lane 9:

Lane 10:

Lane 11:

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 20min

AbReview21275****

Dot Blot - Human Histone H3 (tri methyl K9) peptide (AB1773)

ab1773 (at 0.01 and 0.1 μg) used in Dot Blot to test for cross-reactivity of ab8898 (1/2500). An HRP-conjugated Mouse anti-rabbit polyclonal (1/10000) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Key facts

精製度

>90% HPLC

アプリケーション

applications

生物活性

アクセッション番号

P68431

アニマルフリー

キャリアフリー

種

Human

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

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配列情報

[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":"P68431","tags":[]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

Ambient

長期保存温度

-20°C

False

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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

配列の類似性

Belongs to the histone H3 family.

翻訳後修飾

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.. Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.. Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.. Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication.. Phosphorylated at Thr-4 (H3T3ph) by VRK1 (PubMed:31527692). Phosphorylated at Thr-4 (H3T3ph) by HASPIN during prophase and dephosphorylated during anaphase (PubMed:15681610, PubMed:16185088). Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 or isoform M2 of PKM (PKM2) is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Thr-12 (H3T11ph) by chromatin-associated CHEK1 regulates the transcription of cell cycle regulatory genes by modulating acetylation of Lys-10 (H3K9ac). Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.. Monoubiquitinated at Lys-15 (H3K14ub), Lys-19 (H3K18ub) and Lys-24 (H3K23ub) by UHRF1 and HLTF at hemimethylated DNA sites following DNA replication, promoting recruitment of DNMT1 DNA methyltransferase and replication-coupled DNA methylation maintenance (PubMed:27595565, PubMed:29053958). Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation (PubMed:16678110). This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins (PubMed:16678110).. Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression.. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.. Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis.. Succinylation at Lys-80 (H3K79succ) by KAT2A takes place with a maximum frequency around the transcription start sites of genes (PubMed:29211711). It gives a specific tag for epigenetic transcription activation (PubMed:29211711). Desuccinylation at Lys-123 (H3K122succ) by SIRT7 in response to DNA damage promotes chromatin condensation and double-strand breaks (DSBs) repair (PubMed:27436229).. Serine ADP-ribosylation by PARP1 or PARP2 constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:30257210, PubMed:34874266). Serine ADP-ribosylation at Ser-11 (H3S10ADPr) promotes recruitment of CHD1L (PubMed:34874266). H3S10ADPr is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac) (PubMed:30257210).. Serotonylated by TGM2 at Gln-6 (H3Q5ser) during serotonergic neuron differentiation (PubMed:30867594). H3Q5ser is associated with trimethylation of Lys-5 (H3K4me3) and enhances general transcription factor IID (TFIID) complex-binding to H3K4me3, thereby facilitating transcription (PubMed:30867594).. Dopaminylated by TGM2 at Gln-6 (H3Q5dop) in ventral tegmental area (VTA) neurons (PubMed:32273471). H3Q5dop mediates neurotransmission-independent role of nuclear dopamine by regulating relapse-related transcriptional plasticity in the reward system (By similarity).. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information H3C1 tri methyl K9

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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Epigenetics & chromatin 13:50 PubMed33225957

2020

Histone modifications associated with gene expression and genome accessibility are dynamically enriched at Plasmodium falciparum regulatory sequences.

Applications

Unspecified application

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Jingyi Tang,Scott A Chisholm,Lee M Yeoh,Paul R Gilson,Anthony T Papenfuss,Karen P Day,Michaela Petter,Michael F Duffy

eLife 8: PubMed31573510

2019

H3K9me2 orchestrates inheritance of spatial positioning of peripheral heterochromatin through mitosis.

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Andrey Poleshko,Cheryl L Smith,Son C Nguyen,Priya Sivaramakrishnan,Karen G Wong,John Isaac Murray,Melike Lakadamyali,Eric F Joyce,Rajan Jain,Jonathan A Epstein

Molecular immunology 114:179-188 PubMed31376731

2019

The novel methyltransferase SETD4 regulates TLR agonist-induced expression of cytokines through methylation of lysine 4 at histone 3 in macrophages.

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Yuyun Zhong,Ping Ye,Zhuzhong Mei,Sui Huang,Mengyi Huang,Yue Li,Shixian Niu,Shuqi Zhao,Junwei Cai,Juan Wang,Hequn Zou,Yong Jiang,Jinghua Liu

Cancer research 79:4729-4743 PubMed31308046

2019

SET Domain-Containing Protein 4 Epigenetically Controls Breast Cancer Stem Cell Quiescence.

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Sen Ye,Yan-Fu Ding,Wen-Huan Jia,Xiao-Li Liu,Jing-Yi Feng,Qian Zhu,Sun-Li Cai,Yao-Shun Yang,Qian-Yun Lu,Xue-Ting Huang,Jin-Shu Yang,Sheng-Nan Jia,Guo-Ping Ding,Yue-Hong Wang,Jiao-Jiao Zhou,Yi-Ding Chen,Wei-Jun Yang

The Biochemical journal 476:1753-1769 PubMed31189566

2019

DEK terminates diapause by activation of quiescent cells in the crustacean .

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Species

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Wen-Huan Jia,An-Qi Li,Jing-Yi Feng,Yan-Fu Ding,Sen Ye,Jin-Shu Yang,Wei-Jun Yang

FASEB journal : official publication of the Federa 32:2768-2778 PubMed29295860

2018

Reduced PPARγ2 expression in adipose tissue of male rat offspring from obese dams is associated with epigenetic modifications.

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Species

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Simon Lecoutre,Charlène Pourpe,Laura Butruille,Lucie Marousez,Christine Laborie,Céline Guinez,Jean Lesage,Didier Vieau,Jérôme Eeckhoute,Anne Gabory,Frédérik Oger,Delphine Eberlé,Christophe Breton

Cell 171:573-587.e14 PubMed29033129

2017

Genome-Nuclear Lamina Interactions Regulate Cardiac Stem Cell Lineage Restriction.

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Species

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Andrey Poleshko,Parisha P Shah,Mudit Gupta,Apoorva Babu,Michael P Morley,Lauren J Manderfield,Jamie L Ifkovits,Damelys Calderon,Haig Aghajanian,Javier E Sierra-Pagán,Zheng Sun,Qiaohong Wang,Li Li,Nicole C Dubois,Edward E Morrisey,Mitchell A Lazar,Cheryl L Smith,Jonathan A Epstein,Rajan Jain

Molecular metabolism 6:922-930 PubMed28752055

2017

Maternal obesity programs increased leptin gene expression in rat male offspring via epigenetic modifications in a depot-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Lecoutre,Frederik Oger,Charlène Pourpe,Laura Butruille,Lucie Marousez,Anne Dickes-Coopman,Christine Laborie,Céline Guinez,Jean Lesage,Didier Vieau,Claudine Junien,Delphine Eberlé,Anne Gabory,Jérôme Eeckhoute,Christophe Breton

Molecular and cellular biology 37: PubMed28031330

2017

SETD4 Regulates Cell Quiescence and Catalyzes the Trimethylation of H4K20 during Diapause Formation in Artemia.

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Species

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Li Dai,Sen Ye,Hua-Wei Li,Dian-Fu Chen,Hong-Liang Wang,Sheng-Nan Jia,Cheng Lin,Jin-Shu Yang,Fan Yang,Hiromichi Nagasawa,Wei-Jun Yang

Cancer research 74:3617-3629 PubMed24786786

2014

Loss of the polycomb mark from bivalent promoters leads to activation of cancer-promoting genes in colorectal tumors.

Applications

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Unspecified reactive species

Maria A Hahn,Arthur X Li,Xiwei Wu,Richard Yang,David A Drew,Daniel W Rosenberg,Gerd P Pfeifer

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Histone H3 (tri methyl K9) peptide