AB1342

Human Histone H3 (tri methyl K4) peptide

Name: Human Histone H3 (tri methyl K4) peptide (ab1342) | Abcam
Brand: Abcam Limited
SKU: AB1342
Price: 96000 JPY
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(21 Publications)

Human Histone H3 (tri methyl K4) peptide is a Synthetic blocking peptide. >90% and suitable for Dot, BL.

別名を表示する

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me3, H3K4me, H3K4

17 Images

ChIP - Human Histone H3 (tri methyl K4) peptide (AB1342)

ChIP

Project

ChIP - Human Histone H3 (tri methyl K4) peptide (AB1342)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab78513 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Unknown

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

All lanes:

Western blot - Anti-Histone H3 (di methyl K4, tri methyl K4) antibody [mAbcam 6000] - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-di-methyl-k4-tri-methyl-k4-antibody-mabcam-6000-chip-grade-ab6000'>ab6000</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k27-peptide-ab1782'>ab1782</a>)

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 20min

Project

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

ab78513 is blocked by the addition of Histone H4 (mono methyl K20)peptide (ab17043; lane 6). ab78513 is partially blocked by Histone H4 (di methyl K20) peptide (lane 7).

All lanes:

Western blot - Anti-Histone H4 (mono methyl K20) antibody [5E10-D8] - ChIP Grade (<a href='/products/primary-antibodies/histone-h4-mono-methyl-k20-antibody-5e10-d8-chip-grade-ab78513'>ab78513</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H4 peptide (ab14963)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H4 (mono methyl K20) peptide (ab17043)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Histone H4 peptide (17-24) - di methyl K20

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H4 (tri methyl K20) peptide (<a href='/products/proteins-peptides/human-histone-h4-tri-methyl-k20-peptide-ab17567'>ab17567</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa,17 kDa

true

Exposure time: 1min

Unknown

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

The peptide competition assay shows that this antibody is very specific for Histone H3 di-methylated at K27. It does not recognise Histone H3 mono, di, or tri methylated at K4, Histone H3 mono, di, or tri methylated at K9, or Histone H3 mono, or tri methylated at K27.

All lanes:

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-di-methyl-k27-antibody-chip-grade-ab24684'>ab24684</a>) at 1 µg/mL

Lane 1:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Human Histone H3 peptide (ab17163)

Lane 3:

Calf thymus histone lysate with Human Histone H3 (mono methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf thymus histone lysate with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf thymus histone lysate with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf thymus histone lysate with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf thymus histone lysate with Human Histone H3 (mono methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf thymus histone lysate with Human Histone H3 (di methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf thymus histone lysate with Human Histone H3 (tri methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k27-peptide-ab1782'>ab1782</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG H&L (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 5s

Lab

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

ab78517 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 8 where the activity of ab78517 is specifically blocked by the addition of the immunizing peptide (ab17567).

All lanes:

Western blot - Anti-Histone H4 (di methyl K20, tri methyl K20) antibody [6F8-D9] (<a href='/products/primary-antibodies/histone-h4-di-methyl-k20-tri-methyl-k20-antibody-6f8-d9-ab78517'>ab78517</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 peptide (ab14963)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 (mono methyl K20) peptide (ab17043)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H4 peptide (17-24) - di methyl K20

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 (tri methyl K20) peptide (<a href='/products/proteins-peptides/human-histone-h4-tri-methyl-k20-peptide-ab17567'>ab17567</a>)

Lane 9:

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 11:

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

true

Exposure time: 90s

Unknown

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

ab18521 recognises histone H3 as well as modified histone H3 at approximately 15 kDa(lane1). This is demonstrated by the blocking using the immunising unmodified histone H3 peptide (lane2), histone H3 mono methyl K4 (lane3), histone H3 di methyl K4 (lane4) and histone H3 tri methyl K4 (lane5).

All lanes:

Anti-Histone H3 antibody (ab31602) at 1 µg/mL

Lane 1:

Calf thymus histone lysate at 20 µg

Lane 2:

Calf thymus histone lysate at 20 µg with Human Histone H3 peptide (ab14949)

Lane 3:

Calf thymus histone lysate at 20 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf thymus histone lysate at 20 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf thymus histone lysate at 20 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Project

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

All lanes:

Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-mono-methyl-k9-antibody-chip-grade-ab9045'>ab9045</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Lane 4:

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Lane 7:

Lane 8:

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k27-peptide-ab1782'>ab1782</a>)

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 15 kDa

false

Lab

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

Lane 8 shows that Rabbit polyclonal to Histone H3 (tri methyl K9) is blocked by the addition of the immunizing peptide (ab1773). Cross-reactivity with Histone H3 peptide - tri methyl K27 (ab1782) is also shown in Lane 11.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-tri-methyl-k9-antibody-chip-grade-ab8898'>ab8898</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Lane 9:

Lane 10:

Lane 11:

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

All lanes:

Western blot - HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (<a href='/products/primary-antibodies/hrp-histone-h3-di-methyl-k9-antibody-mabcam-1220-ab62168'>ab62168</a>) at 1/20000 dilution

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2903'>ab2903</a>)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Lane 9:

Lane 10:

Lane 11:

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Lab

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6147 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (<a href='/products/primary-antibodies/histone-h3-di-methyl-k27-tri-methyl-k27-antibody-mabcam-6147-ab6147'>ab6147</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2623'>ab2623</a>)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Lane 9:

Lane 10:

Lane 11:

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 1min

Lab

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab78517).

ab78517 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 8 where the activity of ab78517 is specifically blocked by the addition of the immunizing peptide (ab17567).

All lanes:

Western blot - Anti-Histone H4 (di methyl K20, tri methyl K20) antibody [6F8-D9] - BSA and Azide free (<a href='/products/primary-antibodies/histone-h4-di-methyl-k20-tri-methyl-k20-antibody-6f8-d9-bsa-and-azide-free-ab264528'>ab264528</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 peptide (ab14963)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 (mono methyl K20) peptide (ab17043)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H4 peptide (17-24) - di methyl K20

Lane 9:

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 11:

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

true

Exposure time: 90s

Project

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab12209).

All lanes:

Western blot - Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-tri-methyl-k4-antibody-mabcam12209-chip-grade-ab12209'>ab12209</a>) at 2 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Lane 9:

Lane 10:

Lane 11:

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 16min

Project

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

All lanes:

Western blot - Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h4-mono-methyl-k20-antibody-chip-grade-ab9051'>ab9051</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H4 peptide (ab14963)

Lane 3:

Lane 4:

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Human Histone H4 (mono methyl K20) peptide (ab17043)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg with Histone H4 peptide (17-24) - di methyl K20

Lane 8:

Lane 9:

Lane 10:

Lane 11:

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

false

Exposure time: 10s

Unknown

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

ab8895 is specific for mono-methylated Lysine 4 of histone H3 and does not recognize di- or tri-methyl Lysine 4 nor methylation at Lysine 9. This is shown in lane 2 where the activity of the antibody is specifically blocked by the addition of the immunizing peptide (ab1340).

All lanes:

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-mono-methyl-k4-antibody-chip-grade-ab8895'>ab8895</a>) at 1/500 dilution

Lane 1:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Human Histone H3 (mono methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 3:

Calf thymus histone lysate with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 4:

Calf thymus histone lysate with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 5:

Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 6:

Calf thymus histone lysate with Human Histone H3 (mono methyl K27) peptide (<a href='/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 7:

Calf thymus histone lysate with Human Histone H3 (unmodified ) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2903'>ab2903</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 18 kDa

false

Exposure time: 2min

Project

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

ab9053 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 5 where the activity of ab9053 is specifically blocked by the addition of the immunizing peptide (ab17567).

All lanes:

Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h4-tri-methyl-k20-antibody-chip-grade-ab9053'>ab9053</a>) at 1 µg/mL

Lane 1:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Human Histone H4 peptide (ab2622)

Lane 3:

Calf thymus histone lysate with Human Histone H4 (mono methyl K20) peptide (ab17043)

Lane 4:

Calf thymus histone lysate with Human Histone H4 (di methyl K20) peptide (ab14964)

Lane 5:

Calf thymus histone lysate with Human Histone H4 (tri methyl K20) peptide (<a href='/products/proteins-peptides/human-histone-h4-tri-methyl-k20-peptide-ab17567'>ab17567</a>)

Lane 6:

Calf thymus histone lysate with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 7:

Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

false

Project

Western blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

Expected molecular weights : H3 = 17 kDa; H4 = 14 kDa This image shows that the main epitopes recognized by ab7315 are the di methylated lysine residues. This can be seen in lanes 4, 7 and 10 where the activity of the antibody is quenched by the immunizing peptides (ab7768, ab1772, ab1781).

All lanes:

Western blot - Anti-pan methyl Lysine antibody - ChIP Grade (<a href='/products/primary-antibodies/pan-methyl-lysine-antibody-chip-grade-ab7315'>ab7315</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (ab1342)

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Lane 9:

Lane 10:

Lane 11:

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 14-17 kDa

true

Exposure time: 20min

AbReview21269****

Dot Blot - Human Histone H3 (tri methyl K4) peptide (AB1342)

ab1342 (at 0.01 and 0.1 μg) used in Dot Blot to test for cross-reactivity of ab8580 (1/2500). An HRP-conjugated Mouse anti-rabbit polyclonal (1/10000) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Key facts

精製度

>90% HPLC

アプリケーション

Dot, BL

applications

生物活性

アクセッション番号

P68431

アニマルフリー

キャリアフリー

種

Human

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

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配列情報

[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":"P68431","tags":[]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

Ambient

長期保存温度

-20°C

False

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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

配列の類似性

Belongs to the histone H3 family.

翻訳後修飾

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.. Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.. Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.. Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication.. Phosphorylated at Thr-4 (H3T3ph) by VRK1 (PubMed:31527692). Phosphorylated at Thr-4 (H3T3ph) by HASPIN during prophase and dephosphorylated during anaphase (PubMed:15681610, PubMed:16185088). Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 or isoform M2 of PKM (PKM2) is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Thr-12 (H3T11ph) by chromatin-associated CHEK1 regulates the transcription of cell cycle regulatory genes by modulating acetylation of Lys-10 (H3K9ac). Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.. Monoubiquitinated at Lys-15 (H3K14ub), Lys-19 (H3K18ub) and Lys-24 (H3K23ub) by UHRF1 and HLTF at hemimethylated DNA sites following DNA replication, promoting recruitment of DNMT1 DNA methyltransferase and replication-coupled DNA methylation maintenance (PubMed:27595565, PubMed:29053958). Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation (PubMed:16678110). This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins (PubMed:16678110).. Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression.. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.. Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis.. Succinylation at Lys-80 (H3K79succ) by KAT2A takes place with a maximum frequency around the transcription start sites of genes (PubMed:29211711). It gives a specific tag for epigenetic transcription activation (PubMed:29211711). Desuccinylation at Lys-123 (H3K122succ) by SIRT7 in response to DNA damage promotes chromatin condensation and double-strand breaks (DSBs) repair (PubMed:27436229).. Serine ADP-ribosylation by PARP1 or PARP2 constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:30257210, PubMed:34874266). Serine ADP-ribosylation at Ser-11 (H3S10ADPr) promotes recruitment of CHD1L (PubMed:34874266). H3S10ADPr is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac) (PubMed:30257210).. Serotonylated by TGM2 at Gln-6 (H3Q5ser) during serotonergic neuron differentiation (PubMed:30867594). H3Q5ser is associated with trimethylation of Lys-5 (H3K4me3) and enhances general transcription factor IID (TFIID) complex-binding to H3K4me3, thereby facilitating transcription (PubMed:30867594).. Dopaminylated by TGM2 at Gln-6 (H3Q5dop) in ventral tegmental area (VTA) neurons (PubMed:32273471). H3Q5dop mediates neurotransmission-independent role of nuclear dopamine by regulating relapse-related transcriptional plasticity in the reward system (By similarity).. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information H3C1 tri methyl K4

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文献 (21)

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Human Histone H3 (tri methyl K4) peptide