Human beta Actin peptide (ab13772)
Key features and details
- Purity: > 90% HPLC
- Suitable for: Blocking
製品の詳細
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製品名
Human beta Actin peptide
beta Actin タンパク質・ペプチド 製品一覧 -
精製度
> 90 % HPLC. -
アクセッション番号
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Animal free
No -
由来
Synthetic -
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生物種
Human
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関連製品
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Corresponding Antibody
特性
Our Abpromise guarantee covers the use of ab13772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
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製品の状態
Lyophilized -
備考
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C.
Information available upon request.
関連情報
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別名
- A26C1A
- A26C1B
- ACTB
see all -
機能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
関連疾患
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
配列類似性
Belongs to the actin family. -
翻訳後修飾
ISGylated. -
細胞内局在
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
画像
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Western blot - Human beta Actin peptide (ab13772)All lanes : Anti-beta Actin antibody (ab8227) at 1/1000 dilution
Lane 1 : HeLa nuclear lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK 293 cell lysate
Lane 6 : HeLa nuclear lysate withHuman beta Actin peptide (ab13772) at 1 µg/ml
Lane 7 : HeLa whole cell lysate withHuman beta Actin peptide (ab13772) at 1 µg/ml
Lane 8 : A431 cell lysate withHuman beta Actin peptide (ab13772) at 1 µg/ml
Lane 9 : Jurkate cell lysate withHuman beta Actin peptide (ab13772) at 1 µg/ml
Lane 10 : HEK 293 cell lysate withHuman beta Actin peptide (ab13772) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Exposure time: 5 seconds
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab13772 は 5 報の論文で使用されています。
- Pasquettaz R et al. Peculiar protrusions along tanycyte processes face diverse neural and nonneural cell types in the hypothalamic parenchyma. J Comp Neurol 529:553-575 (2021). PubMed: 32515035
- Carley E et al. The LINC complex transmits integrin-dependent tension to the nuclear lamina and represses epidermal differentiation. Elife 10:N/A (2021). PubMed: 33779546
- Sun D et al. Acanthopanax senticosus polysaccharide suppressing proliferation and metastasis of the human non-small cell lung cancer NCI-H520 cells is associated with Wnt/ß-catenin signaling. Neoplasma 66:555-563 (2019). PubMed: 30943746
- Zeng S et al. Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/ß-catenin pathway. Mol Med Rep 18:5572-5578 (2018). PubMed: 30365080
- Dinter J et al. Different antigen-processing activities in dendritic cells, macrophages, and monocytes lead to uneven production of HIV epitopes and affect CTL recognition. J Immunol 193:4322-34 (2014). PubMed: 25230751
