AB51992

EGFP protein (His tag)

Name: EGFP protein (His tag) (ab51992) | Abcam
Brand: Abcam Limited
SKU: AB51992
Price: 65000 JPY
Availability: InStock

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(1 Publication)

EGFP protein (His tag) is a recombinant Aequorea victoria Full Length protein, in the 2 to 238 aa range, with >=97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, western blot and conjugation assays. The predicted molecular weight of ab51992 protein is 32.7 kDa.

- ideal for use as a positive control
- Contains N-terminal and C-terminal His tags
- Available in different sizes to fit your experimental needs

See more GFP products

別名を表示する

Green fluorescent protein, GFP

Key facts

精製度

>97% SDS-PAGE

エンドトキシンレベル

< 1 EU/µg

発現系

Escherichia coli

アプリケーション

SDS-PAGE, Conjugation, WB

applications

生物活性

アクセッション番号

P42212

アニマルフリー

キャリアフリー

種

Aequorea victoria

再構成

Reconstitute in PBS

バッファー組成

Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Conjugation": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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製品の詳細

Ensure the validity of your result using our EGFP protein (His tag) ab51992 as a control in SDS-PAGE and western blot.

This Enhanced GFP (eGFP) protein is suitable as control reagent for GFP expression studies. Other applications include: calibration of fluorometers and flow cytometers, fluorescence microscopy, and microinjection of GFP into cells and tissues, etc.

The excitation and emmission spectra for this ab51992 recombinant EGFP is Ex/Em = 488/509 nm.

Check out our protein gel staining guide for SDS-PAGE here

Check out our western blot protocol for more information here

This product is manufactured by BioVision, an Abcam company and was previously called 4999 Enhanced Green Fluorescent Protein (EGFP). 4999-100 is the same size as the 100 µg size of ab51992.

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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMASVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKASMTGGQQMGRDPNSSSVDKLAAALEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"32.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":238,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":null,"tags":[{"tag":"His","terminus":"C-Terminus"},{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

製品の状態

Lyophilized

出荷温度

Blue Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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一般的な情報

機能

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

配列の類似性

Belongs to the GFP family.

翻訳後修飾

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information GFP

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 19:5240-5252 PubMed39871506

2025

Large and Stable Nanopores Formed by Complement Component 9 for Characterizing Single Folded Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Wachara Chanakul,Anasua Mukhopadhyay,Saurabh Awasthi,Anna D Protopopova,Alessandro Ianiro,Michael Mayer

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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EGFP protein (His tag)