AGE-BSA (ab51995)
Key features and details
- Expression system: Native
- Purity: > 98% SDS-PAGE
- Suitable for: SDS-PAGE
製品の詳細
-
製品名
AGE-BSA -
精製度
> 98 % SDS-PAGE. -
発現系
Native -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Native -
-
生物種
Cow -
予測される分子量
67 kDa
-
-
製品の詳細
Native Cow AGE protein
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab51995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
-
製品の状態
Liquid -
備考
This product is manufactured by BioVision, an Abcam company and was previously called 2221 AGE-BSA. 2221-10 is the same size as the 10 mg size of ab51995.
May precipitate during extended storage. Sonicate to solubilize the precipitates. The AGE modified BSA was produced by reacting BSA with glycolaldehyde under sterile conditions followed by extensive dialysis and purification steps. Fluorescence of AGEs was confirmed by fluorescence spectrophotometry with Ex./Em. = 370/440 nm. Glycated BSA shows a 7000% increase in fluorescence in compared to control BSA.
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: 98% PBS
関連情報
-
別名
- Advanced glycation end products
- AGEs
-
関連性
The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure. -
細胞内局在
Cell Membrane and Secreted
画像
-
SDS-PAGE - AGE-BSA (ab51995)
4-20% SDS-PAGE of AGE-BSA: 10, 15 and 20 ug of AGE-BSA loaded in each lane under reducing conditions and stained with Coomassie Blue. AGE-BSA has a predicted MW of ~ 66.5 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (25)
ab51995 は 25 報の論文で使用されています。
- Coppola S et al. Increased dietary intake of ultraprocessed foods and mitochondrial metabolism alterations in pediatric obesity. Sci Rep 13:12609 (2023). PubMed: 37537205
- Li Y et al. Resveratrol Alleviates Diabetic Periodontitis-Induced Alveolar Osteocyte Ferroptosis Possibly via Regulation of SLC7A11/GPX4. Nutrients 15:N/A (2023). PubMed: 37432277
- Yang F et al. Advanced Glycation End Products Downregulate Connexin 43 and Connexin 40 in Diabetic Atrial Myocytes via the AMPK Pathway. Diabetes Metab Syndr Obes 16:3045-3056 (2023). PubMed: 37810573
- Gallate ZS et al. Galectin-3 and RAGE differentially control advanced glycation endproduct-induced collagen damage in murine intervertebral disc organ culture. JOR Spine 6:e1254 (2023). PubMed: 37361328
- Qin Y et al. N6-methyladenosine methylation regulator RBM15 promotes the progression of diabetic nephropathy by regulating cell proliferation, inflammation, oxidative stress, and pyroptosis through activating the AGE-RAGE pathway. Environ Toxicol 38:2772-2782 (2023). PubMed: 37551785
