Anti-ZZZ3 抗体 (ab118800)
Key features and details
- Rabbit polyclonal to ZZZ3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZZZ3 antibody -
製品の詳細
Rabbit polyclonal to ZZZ3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Chicken, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide corresponding to a region within amino acids 50-100 of Human ZZZ3 (NP_056349.1).
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ポジティブ･コントロール
- 293T, Jurkat and HeLa whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab118800 was affinity purified using an epitope specific to ZZZ3 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118800の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 102 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 102 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. -
配列類似性
Contains 1 HTH myb-type DNA-binding domain.
Contains 1 ZZ-type zinc finger. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 26009 Human
- SwissProt: Q8IYH5 Human
- Unigene: 480506 Human
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別名
- ATAC component 1 homolog antibody
- ATAC1 antibody
- DKFZp313N0119 antibody
see all
画像
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Western blot - Anti-ZZZ3 antibody (ab118800)All lanes : Anti-ZZZ3 antibody (ab118800) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ZZZ3 antibody (ab118800)Detection of ZZZ3 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118800 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab118800 は 1 報の論文で使用されています。
- Merritt N et al. TAZ-CAMTA1 and YAP-TFE3 alter the TAZ/YAP transcriptome by recruiting the ATAC histone acetyltransferase complex. Elife 10:N/A (2021). PubMed: 33913810