Anti-Zyxin (phospho S142) 抗体 (ab78910)
Key features and details
- Rabbit polyclonal to Zyxin (phospho S142)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Zyxin (phospho S142) antibody
Zyxin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Zyxin (phospho S142) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Zyxin (phospho S142).
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ポジティブ･コントロール
- Extracts from Jurkat cells treated with paclitaxel (1uM, 24hours).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab78910 was purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78910の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 61 kDa.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 61 kDa. |
ターゲット情報
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機能
Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression. -
配列類似性
Belongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains. -
細胞内局在
Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Cell junction, focal adhesion. Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1. - Information by UniProt
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参照データベース
- Entrez Gene: 7791 Human
- Omim: 602002 Human
- SwissProt: Q15942 Human
- Unigene: 490415 Human
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別名
- ESP 2 antibody
- ESP2 antibody
- HED 2 antibody
see all
画像
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Western blot - Anti-Zyxin (phospho S142) antibody (ab78910)All lanes : Anti-Zyxin (phospho S142) antibody (ab78910) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells, treated
with paclitaxel (1uM, 24hours)
Lane 2 : Extracts from Jurkat cells, treated
with paclitaxel (1uM, 24hours) with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab78910 は 1 報の論文で使用されています。
- Bernusso VA et al. Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells. Biochim Biophys Acta 1853:388-95 (2015). PubMed: 25450971