Anti-ZWINT 抗体 (ab84367)
Key features and details
- Rabbit polyclonal to ZWINT
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZWINT antibody
ZWINT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ZWINT -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Macaque monkey
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免疫原
Synthetic peptide corresponding to a region within C terminal amino acids 225-277 (
PEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVN LP
) of Human ZWINT (NP_008988.2) -
ポジティブ･コントロール
- Human testis tissue. HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84367の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/500. Fix with PFA do not fix with acetone.
|
|
| IHC-P |
1/100 - 1/500.
|
| 特記事項 |
|---|
|
ICC/IF
1/100 - 1/500. Fix with PFA do not fix with acetone. |
|
IHC-P
1/100 - 1/500. |
ターゲット情報
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機能
Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. - Information by UniProt
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参照データベース
- Entrez Gene: 11130 Human
- Omim: 609177 Human
- SwissProt: O95229 Human
- Unigene: 591363 Human
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別名
- Human ZW10 interacting protein 1 antibody
- HZwint 1 antibody
- HZwint1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZWINT antibody (ab84367)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human testis tissue using ab84367 at 1/250, followed by DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-ZWINT antibody (ab84367)ab84367 at 1/250 dillution staining ZWINT in NBF-fixed asynchronous human Hela cells by Immunocytochemistry / Immunofluorescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab84367 は 4 報の論文で使用されています。
- Gurden MD et al. Aurora B prevents premature removal of spindle assembly checkpoint proteins from the kinetochore: A key role for Aurora B in mitosis. Oncotarget 9:19525-19542 (2018). PubMed: 29731963
- Faisal A et al. Characterisation of CCT271850, a selective, oral and potent MPS1 inhibitor, used to directly measure in vivo MPS1 inhibition vs therapeutic efficacy. Br J Cancer 116:1166-1176 (2017). PubMed: 28334731
- Brownlow N et al. Mitotic catenation is monitored and resolved by a PKCe-regulated pathway. Nat Commun 5:5685 (2014). IF . PubMed: 25483024
- Genin A et al. Kinetochore KMN network gene CASC5 mutated in primary microcephaly. Hum Mol Genet : (2012). Human . PubMed: 22983954