Anti-ZW10 抗体 (ab21582)
Key features and details
- Rabbit polyclonal to ZW10
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZW10 antibody
ZW10 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ZW10 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IF, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog, Xenopus laevis
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ZW10.
Immunogen の所有権に関して (Peptides available as ab23436, ab23437 and ab23436.) -
ポジティブ･コントロール
- Jurkat whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21582の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
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| ICC/IF | (6) |
1/100.
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| IP |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa). |
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ICC/IF
1/100. |
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IP
Use at an assay dependent concentration. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 9183 Human
- Entrez Gene: 26951 Mouse
- Entrez Gene: 363059 Rat
- Omim: 603954 Human
- SwissProt: O43264 Human
- SwissProt: O54692 Mouse
- SwissProt: Q4V8C2 Rat
- Unigene: 503886 Human
see all -
別名
- Centromere/kinetochore protein zw10 antibody
- Centromere/kinetochore protein zw10 homolog antibody
- HZW 10 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-ZW10 antibody (ab21582)This image is courtesy of Scott Slattery and Mike ManciniPanel one shows staining of HeLa cells by anti-ZW10 (ab21582) in green and DAPI in blue. The second panel shows staining by anti-ZW10 in green and SH-CREST in red, which stains the centromeres. In most cells at interphase, ZW10 is present diffusely in the cytoplasm. In prometaphase, it is associated with the kinetochore. Fix cells for 30 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with 1 mg/ml Na borohydride or 100 mM ammonium chloride in PEM. Permeablize 30 minutes with 0.5% TX-100 in PEM. Block 30 minutes in 5% milk in TBST. Primary antibody incubated overnight at 4oC diluted 1/100 in 5% milk in TBST. Secondary antibody 1 hour at RT diluted in 5% milk in TBST. Post-fix 20 min. on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with ammonium chloride in PEM. Counterstain with DAPI in TBST. Mount with ProLong Gold antifade reagent from Invitrogen. Notes: Ample washing between each step. TBST = Tris buffered saline +
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Western blot - Anti-ZW10 antibody (ab21582)All lanes : Anti-ZW10 antibody (ab21582) at 1 µg/ml
Lane 1 :Jurkat whole cell lysate (ab7899)
Lane 2 :Jurkat whole cell lysate (ab7899) with ZW10 peptide (ab23436) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG(Alexa Fluor® 680) at 1/10,000 dilution
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Additional bands at: 45 kDa (possible cleavage fragment), 45 kDa (possible cross reactivity), 75 kDa (possible cleavage fragment), 75 kDa (possible cross reactivity)
This antibody detects a band at approximately 88kDa that corresponds in size to ZW10. It also detects bands at 75 and 45kDa that may be degradation products or crossreactivity. All of these bands are competed away by the addition of the immunizing peptide, suggesting that they are specific interactions. -
Immunoprecipitation - Anti-ZW10 antibody (ab21582)ZW10 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to ZW10 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab21582.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 88kDa: ZW10 -
Immunocytochemistry/ Immunofluorescence - Anti-ZW10 antibody (ab21582)This image is courtesy of an Abreview submitted by Dr Kirk McManusab21582 (1/200) staining ZW10 in asynchronous mouse 10T1/2 cells (green). Cells were pre-extracted with PEM (1min) at room temperature, fixed in paraformaldehyde (10 mins), washed in KB+ buffer and counterstained with DAPI in order to highlight the DNA/nucleus (red). Please note that ab21582 might only perform well when using this specific protocol. Please refer to abreview for further experimental details.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab21582 は 22 報の論文で使用されています。
- Murillo-Pineda M et al. Induction of spontaneous human neocentromere formation and long-term maturation. J Cell Biol 220:N/A (2021). PubMed: 33443568
- Kumari A et al. Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions. J Cell Biol 220:N/A (2021). PubMed: 34709360
- Rajendraprasad G et al. TH588 and Low-Dose Nocodazole Impair Chromosome Congression by Suppressing Microtubule Turnover within the Mitotic Spindle. Cancers (Basel) 13:N/A (2021). PubMed: 34885104
- Allan LA et al. Cyclin B1 scaffolds MAD1 at the kinetochore corona to activate the mitotic checkpoint. EMBO J 39:e103180 (2020). PubMed: 32202322
- Dang Y et al. Comprehensive analysis of 5-hydroxymethylcytosine in zw10 kinetochore protein as a promising biomarker for screening and diagnosis of early colorectal cancer. Clin Transl Med N/A:N/A (2020). PubMed: 32628818