Anti-ZRANB1 抗体 (ab82132)
Key features and details
- Goat polyclonal to ZRANB1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ZRANB1 antibody
ZRANB1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ZRANB1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow
-
免疫原
Synthetic peptide:
KWLDRYRQIRPCTS
, corresponding to C terminal amino acids 682-695 of Human ZRANB1 -
ポジティブ･コントロール
- Human Cerebellum Lysate
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab82132の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 81 kDa.
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 81 kDa. 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
Positive regulator of the Wnt signaling pathway that specifically cleaves 'Lys-63'-linked ubiquitin chains. Acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription. May also modulate TNF-alpha signaling. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the peptidase C64 family.
Contains 1 OTU domain.
Contains 3 RanBP2-type zinc fingers. -
ドメイン
The OTU domain mediates the deubiquitinating activity.
The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 54764 Human
- Entrez Gene: 360216 Mouse
- Omim: 611749 Human
- SwissProt: Q9UGI0 Human
- SwissProt: Q7M760 Mouse
- Unigene: 595158 Human
- Unigene: 389984 Mouse
- Unigene: 397069 Mouse
-
別名
- DKFZp762P2216 antibody
- hTrabid antibody
- Trabid antibody
see all
画像
-
Western blot - Anti-ZRANB1 antibody (ab82132)Anti-ZRANB1 antibody (ab82132) at 0.1 µg/ml + Human Cerebellum Lysate (35ug in RIPA buffer)
Predicted band size: 81 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab82132 は 1 報の論文で使用されています。
- Li Q et al. Deubiquitinase ZRANB1 drives hepatocellular carcinoma progression through SP1-LOXL2 axis. Am J Cancer Res 11:4807-4825 (2021). PubMed: 34765294