Anti-ZNF462 抗体 (ab117771)
Key features and details
- Rabbit polyclonal to ZNF462
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZNF462 antibody -
製品の詳細
Rabbit polyclonal to ZNF462 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human ZNF462 aa 2450-2550.
Database link: Q96JM2 -
ポジティブ･コントロール
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human lung cancer tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab117771 was affinity purified using an epitope specific to ZNF462 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117771の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 285 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 285 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May be involved in transcriptional regulation. -
配列類似性
Contains 27 C2H2-type zinc fingers. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 58499 Human
- SwissProt: Q96JM2 Human
- Unigene: 370379 Human
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別名
- DKFZp686B2325 antibody
- DKFZp762N2316 antibody
- FLJ14960 antibody
see all
画像
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Western blot - Anti-ZNF462 antibody (ab117771)All lanes : Anti-ZNF462 antibody (ab117771) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK-293T whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 285 kDaSamples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified ab117771 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF462 antibody (ab117771)Immunohistochemical analysis of human lung cancer tissue labeling ZNF462 with ab117771 at a dilution of 1/5000.
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Immunoprecipitation - Anti-ZNF462 antibody (ab117771)Detection of human ZNF462 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified ab117771 used for IP at 6 µg per reaction. For blotting immunoprecipitated ZNF462, ab117771 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Lane 1 : Anti-ZNF462 antibody (ab117771)
Lane 2 : Control IgG
All lanes : -
Western blot - Anti-ZNF462 antibody (ab117771)Anti-ZNF462 antibody (ab117771) at 0.4 µg/ml + NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 285 kDaSamples: Whole cell lysate (50 µg) from NIH/3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified ab117771 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab117771 は 1 報の論文で使用されています。
- Devkota S et al. Functional characterization of EI24-induced autophagy in the degradation of RING-domain E3 ligases. Autophagy 12:2038-2053 (2016). PubMed: 27541728