AB228574

Anti-ZNF45 抗体

Anti-ZNF45 antibody

JP Deleterious:医薬用外劇物

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(1 Publication)

Rabbit Polyclonal ZNF45 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF45.

別名を表示する

KOX5, ZNF13, ZNF45, Zinc finger protein 45, BRC1744, Zinc finger protein 13, Zinc finger protein KOX5

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF45 antibody (AB228574)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF45 antibody (AB228574)

Paraffin-embedded human colon carcinoma tissue stained for ZNF45 with ab228574 at 1/250 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ZNF45 antibody (AB228574)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZNF45 antibody (AB228574)

A375 (human malignant melanoma cell line) cells stained for ZNF45 (green) using ab228574 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain : Hoechst 33342 (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZNF45 antibody (AB228574)

HCT 166 (human colorectal carcinoma cell line) cells stained for ZNF45 (green) using ab228574 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain : Hoechst 33342 (blue).

Supplier Data

Western blot - Anti-ZNF45 antibody (AB228574)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-ZNF45 antibody (ab228574) at 1/2000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Predicted band size: 78 kDa

true

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human ZNF45. The exact immunogen used to generate this antibody is proprietary information.

Q02386

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ZNF45 also known as Zinc Finger Protein 45 belongs to the zinc finger protein family. It acts as a transcription factor and possesses zinc finger domains which helps in DNA binding and regulation of gene expression. The estimated mass of ZNF45 is about 54 kDa. Analysis shows an expression of ZNF45 in various tissues with higher levels observed in specific cell types particularly in the brain and liver. The protein localizes in the nucleus where it directly associates with DNA to exert its functional activities.

Biological function summary

Zinc Finger Protein 45 regulates the expression of genes involved in cell growth and apoptosis. ZNF45 forms part of a larger protein complex which includes other zinc finger proteins and co-factors that modulate its activity. These interactions enable ZNF45 to control various cell processes such as proliferation and differentiation. Through its role in transcription regulation ZNF45 affects the overall cellular homeostasis which helps in maintaining normal cellular functions and responses to environmental signals.

Pathways

The Zinc Finger Protein 45 influences the p53 signaling pathway which plays a critical role in cell cycle regulation and apoptosis. As a transcriptional regulator ZNF45 interacts with other proteins such as MDM2 to modulate the activity of the p53 pathway. Additionally ZNF45 is part of the Wnt signaling pathway where it associates with proteins like β-catenin to regulate the expression of target genes involved in cell cycle progression and oncogenesis. These pathways are essential for maintaining cellular integrity and preventing uncontrolled cell growth.

The altered expression of Zinc Finger Protein 45 associates with several cancers including liver cancer and brain tumors. In liver cancer ZNF45 interacts with proteins such as p53 whose pathway dysregulation can contribute to tumorigenesis. Similarly in brain tumors aberrant expression of ZNF45 may influence pathways involving β-catenin promoting cancer cell survival and proliferation. Understanding the role of ZNF45 in these diseases may provide new insights into potential therapeutic targets for cancer treatment.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May be involved in transcriptional regulation.

See full target information ZNF45

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 16:e15485 PubMed39552193

2024

USP8-mediated PTK7 promotes PIK3CB-related pathway to accelerate the malignant progression of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wencui Kong,Xuegang Feng,Zongyang Yu,Xingfeng Qi,Zhongquan Zhao

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNF45 抗体

Anti-ZNF45 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF45 antibody (AB228574)

Immunocytochemistry/ Immunofluorescence - Anti-ZNF45 antibody (AB228574)

Immunocytochemistry/ Immunofluorescence - Anti-ZNF45 antibody (AB228574)

Western blot - Anti-ZNF45 antibody (AB228574)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise