Anti-ZNF316 抗体 (ab176708)
Key features and details
- Rabbit polyclonal to ZNF316
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZNF316 antibody -
製品の詳細
Rabbit polyclonal to ZNF316 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human ZNF316 aa 475-525. The exact sequence is proprietary.
Sequence:HQAVHTADRPHCCPDCGQAFRLRADFQRHRRGGGCAEAGGDGPRREPGET A
Database link: A6NFI3 -
ポジティブ･コントロール
- HeLa, 293T and Jurkat whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab176708 was affinity purified using an epitope specific to ZNF316 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176708の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 108 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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関連性
May be involved in transcriptional regulation. Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 15 C2H2-type zinc fingers. Contains 1 KRAB domain. -
細胞内局在
Nucleus -
参照データベース
- Entrez Gene: 100131017 Human
- SwissProt: A6NFI3 Human
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別名
- Zinc finger protein 316 antibody
- ZNF316 antibody
画像
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Western blot - Anti-ZNF316 antibody (ab176708)All lanes : Anti-ZNF316 antibody (ab176708) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-ZNF316 antibody (ab176708)ab176708 at 0.4 µg/ml detecting ZNF316 in HeLa whole cell lysate by WB following IP.
Lane 1: ab176708 at 6 µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of ZNF316.
Lane 3: Control IgG.In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab176708 は論文での使用が確認できていません。
