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リコンビナントRabMAb

Anti-ZNF175 抗体 [EPR8391(2)] (ab155075)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] (ab155075)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] (ab155075)
  • Western blot - Anti-ZNF175 antibody [EPR8391(2)] (ab155075)
  • Anti-ZNF175 antibody [EPR8391(2)] (ab155075)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8391(2)] to ZNF175
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ZNF175 antibody [EPR8391(2)]
    ZNF175 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR8391(2)] to ZNF175

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Neuro-2a, SH-SY5Y, 293T, HepG2 whole cell lysate (ab7900); Human colon and pancreas tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab155075の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 82 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000. Predicted molecular weight: 82 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Down-regulates the expression of several chemokine receptors. Interferes with HIV-1 replication by suppressing Tat-induced viral LTR promoter activity.

  • 組織特異性

    Ubiquitous.

  • 配列類似性

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 15 C2H2-type zinc fingers.
    Contains 1 KRAB domain.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • OTK18 antibody
      • Zinc finger protein 175 antibody
      • Zinc finger protein OTK18 antibody
      • ZN175_HUMAN antibody
      • ZNF175 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] (ab155075)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] (ab155075)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling ZNF175 with ab155075 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] (ab155075)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] (ab155075)

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, labeling ZNF175 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-ZNF175 antibody [EPR8391(2)] (ab155075)
      Western blot - Anti-ZNF175 antibody [EPR8391(2)] (ab155075)
      All lanes : Anti-ZNF175 antibody [EPR8391(2)] (ab155075) at 1/100 dilution

      Lane 1 : Neuro-2a cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 82 kDa

    • Anti-ZNF175 antibody [EPR8391(2)] (ab155075)
      Anti-ZNF175 antibody [EPR8391(2)] (ab155075)

    参考文献 (1)

    ab155075 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab155075 は 1 報の論文で使用されています。

    • Liang W  et al. Overexpression of zinc-finger protein 677 inhibits proliferation and invasion by and induces apoptosis in clear cell renal cell carcinoma. Bioengineered 13:5292-5304 (2022). PubMed: 35164660

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