Anti-Zinc finger antiviral protein 抗体 (ab154680)
Key features and details
- Rabbit polyclonal to Zinc finger antiviral protein
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Zinc finger antiviral protein antibody
Zinc finger antiviral protein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Zinc finger antiviral protein -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Zinc finger antiviral protein aa 401-721 (internal sequence).
Database link: Q7Z2W4 -
ポジティブ･コントロール
- A431 whole cell lysate; A431 cells; Human hepatoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154680の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/5000 - 1/20000. Predicted molecular weight: 101 kDa.
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| IHC-P |
1/100 - 1/1000.
|
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| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/5000 - 1/20000. Predicted molecular weight: 101 kDa. |
|
IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Induces an innate immunity to viral infections by preventing the accumulation of viral RNAs in the cytoplasm. Seems to recruit the RNA processing exosome to degrade the target RNAs. Inhibits alphavirus and filovirus replication. -
配列類似性
Contains 4 C3H1-type zinc fingers.
Contains 1 PARP catalytic domain.
Contains 1 WWE domain. -
ドメイン
The second and fourth zinc fingers are involved in binding to specific viral RNAs. -
細胞内局在
Cytoplasm. Nucleus. Localizes in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner. - Information by UniProt
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参照データベース
- Entrez Gene: 56829 Human
- Omim: 607312 Human
- SwissProt: Q7Z2W4 Human
- Unigene: 133512 Human
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別名
- ADP ribosyltransferase diphtheria toxin like 13 antibody
- ARTD13 antibody
- FLB6421 antibody
see all
画像
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Western blot - Anti-Zinc finger antiviral protein antibody (ab154680)Anti-Zinc finger antiviral protein antibody (ab154680) at 1/10000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 101 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Zinc finger antiviral protein antibody (ab154680)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling Zinc finger antiviral protein with ab154680 at 1/200 dilution. Lower panel is co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc finger antiviral protein antibody (ab154680)Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling Zinc finger antiviral protein with ab154680 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab154680 は 14 報の論文で使用されています。
- Lista MJ et al. A Nuclear Export Signal in KHNYN Required for Its Antiviral Activity Evolved as ZAP Emerged in Tetrapods. J Virol 97:e0087222 (2023). PubMed: 36633408
- Nguyen LP et al. Alphavirus Evasion of Zinc Finger Antiviral Protein (ZAP) Correlates with CpG Suppression in a Specific Viral nsP2 Gene Sequence. Viruses 15:N/A (2023). PubMed: 37112813
- Lista MJ et al. Strain-Dependent Restriction of Human Cytomegalovirus by Zinc Finger Antiviral Proteins. J Virol 97:e0184622 (2023). PubMed: 36916924
- Buckmaster MV & Goff SP Riplet Binds the Zinc Finger Antiviral Protein (ZAP) and Augments ZAP-Mediated Restriction of HIV-1. J Virol 96:e0052622 (2022). PubMed: 35913217
- Sungsuwan S et al. Zinc-finger antiviral protein-mediated inhibition of porcine epidemic diarrhea virus growth is antagonized by the coronaviral nucleocapsid protein. Front Microbiol 13:975632 (2022). PubMed: 36160209