AB180574

Anti-Zinc Alpha 2 Glycoprotein 抗体 [EPR14425(B)]

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal Zinc Alpha 2 Glycoprotein antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications.

別名を表示する

ZAG, ZNGP1, AZGP1, Zinc-alpha-2-glycoprotein, Zn-alpha-2-GP, Zn-alpha-2-glycoprotein

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (AB180574)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (AB180574)

Immunohistochemical analysis of Human liver tissue labeling Zinc Alpha 2 Glycoprotein with ab180574 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain : Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (AB180574)

Immunoprecipitation of Human plasma labeling Zinc Alpha 2 Glycoprotein using ab180574at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500.

Blocking/dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/30 dilution

Lane 1:

Human plasma

Lane 2:

Negative control

Secondary

All lanes:

Anti-Rabbit (HRP) specific to the non-reduced form of IgG at 1/500 dilution

Predicted band size: 34 kDa

false

Supplier Data

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (AB180574)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/20000 dilution

All lanes:

Human plasma at 20 µg

Secondary

All lanes:

Anti-rabbit (HRP) specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa

false

Supplier Data

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (AB180574)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/2000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-rabbit HRP at 1/1000 dilution

Predicted band size: 34 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14425(B)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Zinc Alpha 2 Glycoprotein (ZAG) also called ZAG or AZGP1 is a soluble protein with a molecular mass of approximately 41 kDa. It functions as an adipokine and is mostly found in the liver breast and prostate tissues as well as in bodily fluids like plasma and saliva. ZAG belongs to the major histocompatibility complex class I family and it shares structural similarities with antigen-presenting proteins.

Biological function summary

Zinc Alpha 2 Glycoprotein assists in lipid metabolism. It promotes the breakdown of triglycerides in adipose tissue by stimulating the beta-adrenergic pathway. ZAG functions independently and does not typically form complexes with other proteins during this process. Its presence in adipose tissue suggests a role in energy homeostasis and body weight regulation.

Pathways

Zinc Alpha 2 Glycoprotein participates in the metabolic pathway for lipid degradation. The protein interacts with beta-3 adrenergic receptors to trigger lipolysis which involves breaking down fat cells into free fatty acids and glycerol. Its action in this pathway links ZAG with other proteins such as perilipin which protects lipid droplets from breakdown.

Zinc Alpha 2 Glycoprotein has connections with metabolic-related conditions notably obesity and cancer cachexia. In obesity lower levels of ZAG are often observed potentially linking it to impaired fat metabolism. In cancer cachexia elevated ZAG levels are frequently detected suggesting a role in the loss of fat and muscle tissue. ZAG's interaction with certain cytokines may enhance cachexia progression by affecting protein degradation pathways.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.

See full target information AZGP1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:295 PubMed41013181

2025

Secreted AZGP1 induced by 5-FU binds to PD-L1 and promotes apoptosis in cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Eun You,Do Yeon Kim,Hyeseon Yun,Dong-In Koh,Yea Seong Ryu,Dong-Hoon Jin

Open life sciences 19:20221007 PubMed39711976

2024

ZAG promotes colorectal cancer cell proliferation and epithelial-mesenchymal transition by promoting lipid synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Maotao Xu,Xingzheng Jin,Zhouli Shen

Liver international : official journal of the International Association for the Study of the Liver 42:696-709 PubMed34854209

2021

Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Fu,Yixuan Yang,Dazhi Zhang

International journal of molecular sciences 16:691-703 PubMed25561225

2015

Zinc-α-2-glycoprotein: a candidate biomarker for colon cancer diagnosis in Chinese population.

Applications

Species

Human

Yingming Xue,Fudong Yu,Dongwang Yan,Feifei Cui,Huamei Tang,Xiaoliang Wang,Jian Chen,Huijun Lu,Senlin Zhao,Zhihai Peng

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Zinc Alpha 2 Glycoprotein 抗体 [EPR14425(B)]

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (AB180574)

Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (AB180574)

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (AB180574)

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (AB180574)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise