Anti-Zhangfei 抗体 (ab28700)
Key features and details
- Rabbit polyclonal to Zhangfei
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Zhangfei antibody
Zhangfei 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Zhangfei -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Zhangfei.
Immunogen の所有権に関して (Peptide available as ab28713.) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following whole cell lysates: 293T Embryonic Kidney cell line (Human), HepG2 (Human hepatocellular liver carcinoma cell line), MCF7 (Human breast adenocarcinoma cell line), HeLa (Human epithelial carcinoma cell line), PC3 (Human prostate cancer cell line), NIH 3T3 (Mouse embryonic fibroblast cell line). This antibody also gave a positive signal in the following tissue lysates: Human Heart, Human Brain, Human Kidney, Human Liver.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28700の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).
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| 特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa). |
ターゲット情報
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機能
Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells. -
組織特異性
In adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver. -
配列類似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
細胞内局在
Nucleus. Colocalizes at punctate nuclear structures with CREB3 and HCFC1. - Information by UniProt
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参照データベース
- Entrez Gene: 58487 Human
- Entrez Gene: 233490 Mouse
- Omim: 606444 Human
- SwissProt: Q9NS37 Human
- SwissProt: Q91ZR3 Mouse
- Unigene: 535319 Human
- Unigene: 714910 Human
- Unigene: 29496 Mouse
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別名
- 1110034C16Rik antibody
- 6330417B10Rik antibody
- AI225749 antibody
see all
画像
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Western blot - Anti-Zhangfei antibody (ab28700)All lanes : Anti-Zhangfei antibody (ab28700) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lane 5 : 293T Embryonic Kidney cell line (Human) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 9 : PC-3 whole cell lysate (ab3954)
Lane 10 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesIn the literature Zhangfei is also referred to as SMILE (SHP-interacting leucine zipper protein). The SMILE gene generates two isoforms, SMILE-L (long isoform of SMILE, 50–60 kDa) and SMILE-S (short isoform of SMILE, 40 kDa), and ab28700 predominantly recognizes the SMILE-L form.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab28700 は 6 報の論文で使用されています。
- Chen F et al. Activation of CREBZF Increases Cell Apoptosis in Mouse Ovarian Granulosa Cells by Regulating the ERK1/2 and mTOR Signaling Pathways. Int J Mol Sci 19:N/A (2018). PubMed: 30413092
- Chen F et al. Construction and expression of lentiviral vectors encoding recombinant mouse CREBZF in NIH 3T3 cells. Plasmid 76C:24-31 (2014). PubMed: 25195838
- López-Mateo I et al. The transcription factor CREBZF is a novel positive regulator of p53. Cell Cycle 11:3887-95 (2012). PubMed: 22983008
- Misra J et al. Curcumin Differentially Regulates Endoplasmic Reticulum Stress through Transcriptional Corepressor SMILE (Small Heterodimer Partner-interacting Leucine Zipper Protein)-mediated Inhibition of CREBH (cAMP Responsive Element-binding Protein H). J Biol Chem 286:41972-84 (2011). WB . PubMed: 21994947
- Xie YB et al. Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation. J Biol Chem 284:28762-74 (2009). WB ; Human . PubMed: 19690166
- Xie YB et al. SMILE, a new orphan nuclear receptor SHP-interacting protein, regulates SHP-repressed estrogen receptor transactivation. Biochem J 416:463-73 (2008). WB ; Human . PubMed: 18657049