Anti-Zhangfei 抗体

• WB

Project

Western blot - Anti-Zhangfei antibody (AB28700)

In the literature Zhangfei is also referred to as SMILE (SHP-interacting leucine zipper protein). The SMILE gene generates two isoforms, SMILE-L (long isoform of SMILE, 50–60 kDa) and SMILE-S (short isoform of SMILE, 40 kDa), and ab28700 predominantly recognizes the SMILE-L form.

All lanes:

Western blot - Anti-Zhangfei antibody (ab28700) at 1 µg/mL

Lane 1:

Human heart tissue lysate - total protein (ab29431) at 20 µg

Lane 2:

Human brain tissue lysate - total protein (ab29466) at 20 µg

Lane 3:

Human kidney tissue lysate - total protein (ab30203) at 20 µg

Lane 4:

Human liver tissue lysate - total protein (ab29889) at 20 µg

Lane 5:

293T Embryonic Kidney cell line (Human) Whole Cell Lysate at 20 µg

Lane 6:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 7:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

Lane 8:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 9:

PC-3 whole cell lysate (ab3954) at 20 µg

Lane 10:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 37 kDa

Observed band size: 50 kDa

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Exposure time: 2min