AB45341

Anti-Zfp57 抗体

Anti-Zfp57 antibody

(2 Reviews)

(19 Publications)

Rabbit Polyclonal ZFP57 antibody. Suitable for WB and reacts with Mouse samples. Cited in 19 publications.

別名を表示する

Zinc finger protein 57, Zfp-57, Zfp57

1 Images

Collaborator

Western blot - Anti-Zfp57 antibody (AB45341)

All lanes:

Western blot - Anti-Zfp57 antibody (ab45341) at 1/1000 dilution

Lanes 1 - 2:

Wild type mouse embryonic stem cell lysate

Lane 3:

Zfp57 knockout mouse embryonic stem cell lysate

Secondary

All lanes:

Goat anti Rabbit HRP at 1/3000 dilution

Predicted band size: 52 kDa

true

Image courtesy of Dr Nozomi Takahashi, Ferguson-Smith Lab, University of Cambridge.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein Zfp57 also known as Zinc finger protein 57 plays a significant mechanical role in the regulation of gene expression. It acts as a transcriptional repressor by binding to DNA methylated sites using its zinc finger domains to recognize specific DNA sequences. The molecular mass of Zfp57 is approximately 58 kDa. Zfp57 is generally expressed in early embryonic stem cells and in the developing mouse embryo indicating a role in early development.

Biological function summary

Zfp57 maintains DNA methylation at imprinted genes essential for genomic imprinting stability. This protein forms a complex with other proteins including TRIM28 (also known as KAP1) which facilitates its function in establishing and maintaining DNA methylation patterns. By interacting with these partners Zfp57 ensures proper transmission of epigenetic marks across generations.

Pathways

The activity of Zfp57 associates with the epigenetic regulation pathway particularly playing a role in the maintenance of DNA methylation imprints. It directly interacts with methyl-DNA binding proteins such as MeCP2 which are important in interpreting methylation signals to adjust chromatin conformation. The proper function of Zfp57 within these pathways is necessary for maintaining normal genomic architecture.

Alterations in Zfp57 function could lead to problems like transient neonatal diabetes mellitus and related imprinting disorders. These conditions arise when there is improper methylation imprint maintenance due to Zfp57 malfunction. Moreover the protein connects to other factors like NLRP7 influencing the risk of imprinting disorders that affect normal growth and development during infancy.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Transcription regulator required to maintain maternal and paternal gene imprinting, a process by which gene expression is restricted in a parent of origin-specific manner by epigenetic modification of genomic DNA and chromatin, including DNA methylation. Acts by controlling DNA methylation during the earliest multicellular stages of development at multiple imprinting control regions (ICRs) (PubMed : 15070898, PubMed : 18854139, PubMed : 23059534, PubMed : 30602440). Acts together with ZNF445, but ZFP57 plays the predominant role in imprinting maintenance. In contrast, in humans, ZNF445 seems to be the major factor early embryonic imprinting maintenance (PubMed : 30602440). Required for the establishment of maternal methylation imprints at SNRPN locus. Acts as a transcriptional repressor in Schwann cells. Binds to a 5'-TGCCGC-3' consensus sequence and recognizes the methylated CpG within this element (PubMed : 15070898, PubMed : 18854139, PubMed : 23059534).

See full target information Zinc finger protein 57

style

left-column

文献 (19)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112380 PubMed37061916

2023

Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures.

Applications

Unspecified application

Species

Unspecified reactive species

Jack-Christophe Cossec,Tatiana Traboulsi,Sébastien Sart,Yann Loe-Mie,Manuel Guthmann,Ivo A Hendriks,Ilan Theurillat,Michael L Nielsen,Maria-Elena Torres-Padilla,Charles N Baroud,Anne Dejean

Epigenetics & chromatin 15:27 PubMed35918739

2022

The mismatch-repair proteins MSH2 and MSH6 interact with the imprinting control regions through the ZFP57-KAP1 complex.

Applications

Unspecified application

Species

Unspecified reactive species

Basilia Acurzio,Francesco Cecere,Carlo Giaccari,Ankit Verma,Rosita Russo,Mariangela Valletta,Bruno Hay Mele,Claudia Angelini,Angela Chambery,Andrea Riccio

Nucleic acids research 50:5080-5094 PubMed35544282

2022

Epigenome editing reveals core DNA methylation for imprinting control in the Dlk1-Dio3 imprinted domain.

Applications

Unspecified application

Species

Unspecified reactive species

Shin Kojima,Naoya Shiochi,Kazuki Sato,Mamiko Yamaura,Toshiaki Ito,Nodoka Yamamura,Naoki Goto,Mika Odamoto,Shin Kobayashi,Tohru Kimura,Yoichi Sekita

iScience 25:103541 PubMed34977504

2022

β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy LeBlanc,Mijeong Kim,Aparna Kambhampati,Albert J Son,Nereida Ramirez,Jonghwan Kim

Protein & cell 13:102-119 PubMed34865203

2021

Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongling Zhang,Yuanyuan Li,Yongjian Ma,Chongping Lai,Qian Yu,Guangyong Shi,Jinsong Li

Molecular cell 81:2944-2959.e10 PubMed34166609

2021

Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Lenka Skalska,Victoria Begley,Manuel Beltran,Saulius Lukauskas,Garima Khandelwal,Peter Faull,Amandeep Bhamra,Manuel Tavares,Rachel Wellman,Andrey Tvardovskiy,Benjamin M Foster,Igor Ruiz de Los Mozos,Javier Herrero,Silvia Surinova,Ambrosius P Snijders,Till Bartke,Richard G Jenner

Human molecular genetics 30:1509-1520 PubMed34132339

2021

The number of the CTCF binding sites of the H19/IGF2:IG-DMR correlates with DNA methylation and expression imprinting in a humanized mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Freschi,Rosita Del Prete,Laura Pignata,Francesco Cecere,Francesco Manfrevola,Monica Mattia,Gilda Cobellis,Angela Sparago,Marisa S Bartolomei,Andrea Riccio,Flavia Cerrato

Journal of advanced research 33:81-98 PubMed34603780

2021

Offspring production of ovarian organoids derived from spermatogonial stem cells by defined factors with chromatin reorganization.

Applications

Unspecified application

Species

Unspecified reactive species

Huacheng Luo,Xiaoyong Li,Geng G Tian,Dali Li,Changliang Hou,Xinbao Ding,Lin Hou,Qifeng Lyu,Yunze Yang,Austin J Cooney,Wenhai Xie,Ji Xiong,Hu Wang,Xiaodong Zhao,Ji Wu

Human molecular genetics 30:564-574 PubMed33709141

2021

Humanization of a tandem repeat in IG-DMR causes stochastic restoration of paternal imprinting at mouse Dlk1-Dio3 domain.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Hara,Miho Terao,Atsumi Tsuji-Hosokawa,Yuya Ogawa,Shuji Takada

Epigenetics & chromatin 11:36 PubMed29958543

2018

Synthetic DNA fragments bearing ICR cis elements become differentially methylated and recapitulate genomic imprinting in transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hitomi Matsuzaki,Eiichi Okamura,Daichi Kuramochi,Aki Ushiki,Katsuhiko Hirakawa,Akiyoshi Fukamizu,Keiji Tanimoto

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Zfp57 抗体

Anti-Zfp57 antibody

Western blot - Anti-Zfp57 antibody (AB45341)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (19)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise