Anti-Zebrafish Gut Secretory Cell Epitopes 抗体 [FIS 2F11/2] (ab71286)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] (ab71286)](https://www.abcam.co.jp/ps/products/71/ab71286/Images/ab71286-343240-AP2968146ZFlarvaewhole05jpg-x20.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] (ab71286)")
Key features and details
- Mouse monoclonal [FIS 2F11/2] to Zebrafish Gut Secretory Cell Epitopes
- Suitable for: IHC-P
- Reacts with: Zebrafish
- Isotype: IgG1
製品の概要
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製品名
Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] -
製品の詳細
Mouse monoclonal [FIS 2F11/2] to Zebrafish Gut Secretory Cell Epitopes -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Zebrafish -
免疫原
Tissue, cells or virus corresponding to Zebrafish Zebrafish Gut Secretory Cell Epitopes. Tissue/cell preparation: Lysate of zebrafish intestine.
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ポジティブ･コントロール
- IHC: Zebrafish Larvae (5 days)
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
FIS 2F11/2 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71286の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 0.5 µg/ml.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 0.5 µg/ml. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] (ab71286)
IHC image of Zebrafish Gut Secretory Cell Epitopes staining in a section of formalin-fixed paraffin-embedded Zebra fish Larvae (5 days) performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab71286, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (35)
ab71286 は 35 報の論文で使用されています。
- Karolczak S et al. Loss of Mtm1 causes cholestatic liver disease in a model of X-linked myotubular myopathy. J Clin Invest 133:N/A (2023). PubMed: 37490339
- Song J et al. The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathway. NPJ Regen Med 8:20 (2023). PubMed: 37024481
- Ali RQ et al. Loss of zebrafish pkd1l1 causes biliary defects that have implications for biliary atresia splenic malformation. Dis Model Mech 16:N/A (2023). PubMed: 37675454
- Yang D et al. Roxadustat induces hepatotoxicity in zebrafish embryos via inhibiting Notch signaling. J Appl Toxicol 43:1073-1082 (2023). PubMed: 36755374
- Zhao C et al. Regenerative failure of intrahepatic biliary cells in Alagille syndrome rescued by elevated Jagged/Notch/Sox9 signaling. Proc Natl Acad Sci U S A 119:e2201097119 (2022). PubMed: 36469766