AB155249

Anti-ZEB1 抗体

Name: Anti-ZEB1 抗体 (ab155249) | Abcam
Brand: Abcam Limited
SKU: AB155249
Price: 76000 JPY
Availability: InStock

Anti-ZEB1 antibody

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(19 Publications)

Rabbit Polyclonal ZEB1 antibody. Suitable for IP, ChIP, WB, ICC/IF and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZEB1 aa 250 to C-terminus.

別名を表示する

AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (AB155249)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (AB155249)

Immunofluorescence analysis of 4% paraformaldehyde fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling ZEB1 at nucleus (green) with ab155249 at 1/500 dilution. Red : Phalloidin, a cytoskeleton marker, at 1/100 dilution.

Scale bar = 10 μm.

Supplier Data

Immunoprecipitation - Anti-ZEB1 antibody (AB155249)

IP Sample : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.

A - 40 μg of HEK-293T whole cell lysate.

B - Control with 2 μg of preimmune rabbit IgG.

C - Immunoprecipitation of ZEB1 protein by 2 μg of ab155249.

The immunoprecipitated ZEB1 protein was detected by ab155249 diluted at 1 : 1000, followed by anti-rabbit IgG.

7.5% SDS-PAGE

All lanes:

Immunoprecipitation - Anti-ZEB1 antibody (ab155249)

Predicted band size: 124 kDa

false

ChIP

Supplier Data

ChIP - Anti-ZEB1 antibody (AB155249)

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or ab155249. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.

ChIP experiment and primer designs are based on Proc Natl Acad Sci U S A. 2011 Nov 29;108(48) : 19204-9.

Supplier Data

Western blot - Anti-ZEB1 antibody (AB155249)

Samples were separated by 5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. The observed M.W. is based on the publication : PMID : 19706487.

All lanes:

Western blot - Anti-ZEB1 antibody (ab155249) at 1/1000 dilution

Lane 1:

293T Whole cell extract at 30 µg

Lane 2:

A431 Whole cell extract at 30 µg

Lane 3:

HeLa Whole cell extract at 30 µg

Lane 4:

HepG2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 124 kDa

false

Supplier Data

Western blot - Anti-ZEB1 antibody (AB155249)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ZEB1 antibody (ab155249) at 1/500 dilution

Lane 1:

Non-transfected (-) HeLa whole cell extracts at 30 µg

Lane 2:

Transfected (+) HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 124 kDa

true

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human, Rat

アプリケーション

ICC/IF, ChIP, WB, IP

applications

免疫原

Recombinant Fragment Protein within Human ZEB1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P37275

style

left-column