Anti-ZEB1 抗体 [2A8A6] (ab181451)
![Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.co.jp/ps/products/181/ab181451/Images/ab181451-298747-anti-zeb1-antibody-2a8a6-western-blot.jpg "Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)")
Key features and details
- Mouse monoclonal [2A8A6] to ZEB1
- Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-ZEB1 antibody [2A8A6]
ZEB1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2A8A6] to ZEB1 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, Flow Cyt, IHC-P, WBmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human ZEB1 aa 967-1108. Expressed in E. coli.
Sequence:KRFS HSGSYSQHMN HRYSYCKREA EERDSTEQEE AGPEILSNEH VGARASPSQG DSDERESLTR EEDEDSEKEE EEEDKEMEEL QEEKECEKPQ GDEEEEEEEE EVEEEEVEEA ENEGEEAKTE GLMKDDRAES QASSLGQK
Database link: P37275 -
ポジティブ･コントロール
- WB: Human ZEB1 recombinant protein and HAP1 cell lysate. IHC-P: Human cervical cancer and rectum cancer tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR224480-10 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% protein stabilizer consisting of amino acid =85% pH(1% water solution) =7.0~7.5 Water =2% As(mg/kg) =0.5 Pb(mg/kg) =0.1 -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
2A8A6 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181451の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/200 - 1/1000.
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| Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
1/200 - 1/1000.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 124 kDa.
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| 特記事項 |
|---|
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ICC/IF
1/200 - 1/1000. |
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Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P
1/200 - 1/1000. |
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WB
1/500 - 1/2000. Predicted molecular weight: 124 kDa. |
ターゲット情報
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機能
Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. -
組織特異性
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. -
関連疾患
Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. -
配列類似性
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Contains 7 C2H2-type zinc fingers.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6935 Human
- Omim: 189909 Human
- SwissProt: P37275 Human
- Unigene: 124503 Human
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別名
- AREB 6 antibody
- AREB6 antibody
- BZP antibody
see all
画像
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Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ZEB1 knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab181451 observed at 200 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab181451 was shown to recognize ZEB1 in wild-type HAP1 cells as signal was lost at the expected MW in ZEB1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ZEB1 knockout samples were subjected to SDS-PAGE. Ab181451 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.co.jp/ps/products/181/ab181451/Images/ab181451-211157-anti-zeb1-antibody-2a8a6-western-blot.jpg)
Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)All lanes : Anti-ZEB1 antibody [2A8A6] (ab181451) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : KEB1 (AA: 967-1108)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 124 kDa -
![Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.co.jp/ps/products/181/ab181451/Images/ab181451-211156-anti-zeb1-antibody-2a8a6-western-blot.jpg)
Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)Anti-ZEB1 antibody [2A8A6] (ab181451) at 1/500 dilution + ZEB1 recombinant protein fragment (Expected MW is 41.7 kDa)
Predicted band size: 124 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.co.jp/ps/products/181/ab181451/Images/ab181451-211155-anti-zeb1-antibody-2a8a6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [2A8A6] (ab181451)Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling ZEB1 with ab181451 at 1/200 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.co.jp/ps/products/181/ab181451/Images/ab181451-211153-anti-zeb1-antibody-2a8a6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [2A8A6] (ab181451)Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling ZEB1 with ab181451 at 1/200 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.co.jp/ps/products/181/ab181451/Images/ab181451-211152-anti-zeb1-antibody-2a8a6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody [2A8A6] (ab181451)Immunofluorescence analysis of HeLa cells labeling ZEB1 (green) with ab181451 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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![Flow Cytometry - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.co.jp/ps/products/181/ab181451/Images/ab181451-211150-anti-zeb1-antibody-2a8a6-flow-cytometry.jpg)
Flow Cytometry - Anti-ZEB1 antibody [2A8A6] (ab181451)Flow cytometric analysis of HeLa cells labeling ZEB1 (green) with ab181451 at 1/200 dilution. Negative control (red).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab181451 は 19 報の論文で使用されています。
- Chen Y et al. LncRNA DIO3OS regulated by TGF-ß1 and resveratrol enhances epithelial mesenchymal transition of benign prostatic hyperplasia epithelial cells and proliferation of prostate stromal cells. Transl Androl Urol 10:643-653 (2021). PubMed: 33718067
- Kim CY et al. HOXA5 confers tamoxifen resistance via the PI3K/AKT signaling pathway in ER-positive breast cancer. J Cancer 12:4626-4637 (2021). PubMed: 34149926
- Zhao L et al. Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis. Aging (Albany NY) 12:2974-2991 (2020). PubMed: 32039833
- Ma C et al. CircRNA_ACAP2 Suppresses EMT in Head and Neck Squamous Cell Carcinoma by Targeting the miR-21-5p/STAT3 Signaling Axis. Front Oncol 10:583682 (2020). PubMed: 33363013
- Chi G et al. Silencing hsa_circ_PVT1 (circPVT1) suppresses the growth and metastasis of glioblastoma multiforme cells by up-regulation of miR-199a-5p. Artif Cells Nanomed Biotechnol 48:188-196 (2020). PubMed: 31865777