Anti-ZCCHC8 抗体 (ab68739)
Key features and details
- Mouse polyclonal to ZCCHC8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ZCCHC8 antibody
ZCCHC8 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to ZCCHC8 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human ZCCHC8. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- ZCCHC8 transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68739の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 79 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 79 kDa). |
ターゲット情報
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機能
May be involved in pre-mRNA splicing. -
配列類似性
Belongs to the ZCCHC8 family.
Contains 1 CCHC-type zinc finger. - Information by UniProt
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参照データベース
- Entrez Gene: 55596 Human
- SwissProt: Q6NZY4 Human
- Unigene: 37706 Human
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別名
- DKFZp434E2220 antibody
- ZCCHC8 antibody
- ZCHC8_HUMAN antibody
see all
画像
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Western blot - Anti-ZCCHC8 antibody (ab68739)All lanes : Anti-ZCCHC8 antibody (ab68739) at 1/500 dilution
Lane 1 : ZCCHC8 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 79 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (5)
ab68739 は 5 報の論文で使用されています。
- Puno MR & Lima CD Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex. Cell 185:2132-2147.e26 (2022). PubMed: 35688134
- Lloret-Llinares M & Jensen TH Global Identification of Human Exosome Substrates Using RNA Interference and RNA Sequencing. Methods Mol Biol 2062:127-145 (2020). PubMed: 31768975
- Gable DL et al. ZCCHC8, the nuclear exosome targeting component, is mutated in familial pulmonary fibrosis and is required for telomerase RNA maturation. Genes Dev 33:1381-1396 (2019). PubMed: 31488579
- Garland W et al. A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2. Cell Rep 29:1800-1811.e6 (2019). PubMed: 31722198
- Falk S et al. Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors. Nat Commun 7:13573 (2016). WB . PubMed: 27905398
