Anti-ZBTB40 抗体 (ab86330)
Key features and details
- Rabbit polyclonal to ZBTB40
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZBTB40 antibody
ZBTB40 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ZBTB40 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region between residues 1140 and 1190 of human ZBTB40 (NP_055685.3)
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ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab86330 was affinity purified using an epitope specific to ZBTB40 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86330の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 138 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 138 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
May be involved in transcriptional regulation. -
配列類似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 12 C2H2-type zinc fingers. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9923 Human
- Omim: 612106 Human
- SwissProt: Q9NUA8 Human
- Unigene: 418966 Human
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別名
- BC059177 antibody
- C230087D24 antibody
- Gm571 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB40 antibody (ab86330)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZBTB40 with ab86330 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-ZBTB40 antibody (ab86330)All lanes : Anti-ZBTB40 antibody (ab86330) at 0.1 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : 293T whole cell lysates at 5 µg
Developed using the ECL technique.
Predicted band size: 138 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ZBTB40 antibody (ab86330)Detection of ZBTB40 by Western Blot of Immunprecipitate.
ab86330, at 1 µg/ml, staining ZBTB40 in HeLa whole cell lysate immunoprecipitated using ab86330 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 3 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab86330 は論文での使用が確認できていません。