Anti-ZBT10 抗体 (ab117786)
Key features and details
- Rabbit polyclonal to ZBT10
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZBT10 antibody -
製品の詳細
Rabbit polyclonal to ZBT10 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Cow, Pig, Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region within amino acids 50-100 of Human ZBT10 (NP_076418.3).
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ポジティブ･コントロール
- Jurkat and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab117786 was affinity purified using an epitope specific to ZBT10 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117786の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 95 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
May be involved in transcriptional regulation. -
配列類似性
Contains 1 BTB (POZ) domain.
Contains 2 C2H2-type zinc fingers. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 65986 Human
- SwissProt: Q96DT7 Human
- Unigene: 591868 Human
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別名
- FLJ12752 antibody
- OTTHUMP00000207119 antibody
- RINZF antibody
see all
画像
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Western blot - Anti-ZBT10 antibody (ab117786)All lanes : Anti-ZBT10 antibody (ab117786) at 0.1 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : Jurkat whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 95 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-ZBT10 antibody (ab117786)Detection of ZBT10 by Western Blot of Immunprecipitate.
ab117786 at 1µg/ml staining ZBT10 in 293T whole cell lysate immunoprecipitated using ab117786 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab117786 は 2 報の論文で使用されています。
- Fosso E et al. Quercetin's Dual Mode of Action to Counteract the Sp1-miR-27a Axis in Colorectal Cancer Cells. Antioxidants (Basel) 12:N/A (2023). PubMed: 37627542
- Wen YC et al. Pyruvate kinase L/R links metabolism dysfunction to neuroendocrine differentiation of prostate cancer by ZBTB10 deficiency. Cell Death Dis 13:252 (2022). PubMed: 35306527