Anti-ZAK 抗体 - N-terminal (ab154602)
Key features and details
- Rabbit polyclonal to ZAK - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZAK antibody - N-terminal
ZAK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ZAK - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow
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免疫原
Recombinant fragment corresponding to a region within N terminal amino acids 1-259 of Human ZAK.
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ポジティブ･コントロール
- 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji cell lysates; A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154602の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 91 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 91 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'. -
組織特異性
Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
細胞内局在
Cytoplasm. Nucleus. Translocates to the nucleus upon ultraviolet B irradiation. - Information by UniProt
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参照データベース
- Entrez Gene: 519251 Cow
- Entrez Gene: 51776 Human
- Omim: 609479 Human
- SwissProt: Q9NYL2 Human
- Unigene: 444451 Human
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別名
- AZK antibody
- Cervical cancer suppressor gene 4 protein antibody
- HCCS 4 antibody
see all
画像
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Western blot - Anti-ZAK antibody - N-terminal (ab154602)Anti-ZAK antibody - N-terminal (ab154602) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size: 91 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ZAK antibody - N-terminal (ab154602)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling ZAK with ab154602 at 1/200 dilution. Lower panel is merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154602 は論文での使用が確認できていません。