Anti-YY1 抗体 [EPR4651] (ab109228)
![Western blot - Anti-YY1 antibody [EPR4651] (ab109228)](https://www.abcam.co.jp/ps/products/109/ab109228/Images/ab109228-121895-anti-yy1-antibody-epr4651-western-blot.jpg "Western blot - Anti-YY1 antibody [EPR4651] (ab109228)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4651] to YY1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-YY1 antibody [EPR4651]
YY1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4651] to YY1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human colon tissue; HeLa, Y79 and HuT-78 cell lysates
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4651 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109228の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
追加情報
Is unsuitable for ICC/IF.
ターゲット情報
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機能
Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. -
配列類似性
Belongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers. -
細胞内局在
Nucleus matrix. Associated with the nuclear matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 7528 Human
- Entrez Gene: 22632 Mouse
- Entrez Gene: 24919 Rat
- Omim: 600013 Human
- SwissProt: P25490 Human
- SwissProt: Q00899 Mouse
- Unigene: 388927 Human
- Unigene: 3868 Mouse
see all -
別名
- CF1 antibody
- Delta antibody
- Delta transcription factor antibody
see all
画像
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Western blot - Anti-YY1 antibody [EPR4651] (ab109228)All lanes : Anti-YY1 antibody [EPR4651] (ab109228) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Y79 cell lysates
Lane 3 : HuT-78 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4651] (ab109228)](https://www.abcam.co.jp/ps/products/109/ab109228/Images/ab109228-121894-anti-yy1-antibody-epr4651-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4651] (ab109228)Immunohistochemical analysis of YY1 in paraffin embedded Human colon tissue, using a 1/250 dilution of ab109228.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-YY1 antibody [EPR4651] (ab109228)](https://www.abcam.co.jp/ps/products/109/ab109228/Images/ab109228-3-benefits-of-recombinant-antibodies.png)
Anti-YY1 antibody [EPR4651] (ab109228)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab109228 は 28 報の論文で使用されています。
- Morinaka T et al. RYBP contributes to improved prognosis in colorectal cancer via regulation of cell cycle, apoptosis and oxaliplatin sensitivity. Int J Oncol 63:N/A (2023). PubMed: 37654197
- Hu Y et al. RBM14 as a novel epigenetic-activated tumor oncogene is implicated in the reprogramming of glycolysis in lung cancer. World J Surg Oncol 21:132 (2023). PubMed: 37060064
- Alblihy A et al. Selective Killing of BRCA2-Deficient Ovarian Cancer Cells via MRE11 Blockade. Int J Mol Sci 24:N/A (2023). PubMed: 37446144
- Zhang J et al. Disruption of RCAN1.4 expression mediated by YY1/HDAC2 modulates chronic renal allograft interstitial fibrosis. Cell Death Discov 9:271 (2023). PubMed: 37507403
- Chen X et al. TTN-AS1 accelerates the growth and migration of nasopharyngeal carcinoma cells via targeting miR-876-5p/NETO2. Mol Ther Oncolytics 24:535-546 (2022). PubMed: 35229031