Anti-YY1 抗体 (ab245365)
Key features and details
- Rabbit polyclonal to YY1
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-YY1 antibody
YY1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to YY1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human YY1 aa 50-100. The exact sequence is proprietary. NP_003394.1
Database link: P25490 -
ポジティブ･コントロール
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma and mouse teratoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245365 was affinity purified using an epitope specific to YY1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245365の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 45 kDa. |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. -
配列類似性
Belongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers. -
細胞内局在
Nucleus matrix. Associated with the nuclear matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 7528 Human
- Entrez Gene: 22632 Mouse
- Omim: 600013 Human
- SwissProt: P25490 Human
- SwissProt: Q00899 Mouse
- Unigene: 388927 Human
- Unigene: 3868 Mouse
- Unigene: 458511 Mouse
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別名
- CF1 antibody
- Delta antibody
- Delta transcription factor antibody
see all
画像
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Western blot - Anti-YY1 antibody (ab245365)All lanes : Anti-YY1 antibody (ab245365) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-YY1 antibody (ab245365)YY1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245365 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245365 at 1 µg/ml.
Lane 1: ab245365 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody (ab245365)Formalin-fixed, paraffin-embedded human breast carcinoma tissue (left) and mouse teratoma tissue (right) stained for YY1 using ab245365 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab245365 は 3 報の論文で使用されています。
- Lu C et al. Identification of CXCL10 and CXCL11 as the candidate genes involving the development of colitis-associated colorectal cancer. Front Genet 13:945414 (2022). PubMed: 36003333
- Bergamini CM et al. Inhibition of the lncRNA Coded within Transglutaminase 2 Gene Impacts Several Relevant Networks in MCF-7 Breast Cancer Cells. Noncoding RNA 7:N/A (2021). PubMed: 34449674
- Bianchi N et al. The Motility and Mesenchymal Features of Breast Cancer Cells Correlate with the Levels and Intracellular Localization of Transglutaminase Type 2. Cells 10:N/A (2021). PubMed: 34831282