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リコンビナントRabMAb

Anti-YTHDF1 + YTHDF3 + YTHDF2 抗体 [EPR26183-69] (ab290734)

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Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
  • Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
  • Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
  • Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
  • Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
  • Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
  • Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
  • Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
  • Anti-YTHDF1 antibody [EPR26183-69] (ab290734)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26183-69] to YTHDF1 + YTHDF3 + YTHDF2
  • Suitable for: WB, IP, Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69]

  • 製品の詳細

    Rabbit monoclonal [EPR26183-69] to YTHDF1 + YTHDF3 + YTHDF2

  • 由来種

    Rabbit

  • 特異性

    Please note that this antibody does not react with Rat species for ICC and Flow Cyt (intra) applications.

  • アプリケーション

    適用あり: WB, IP, Flow Cyt (Intra), ICC/IFmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa (human cervix adenocarcinoma epithelial cell) and 293T (human embryonic kidney epithelial cell) whole cell lysates. NIH/3T3 (mouse embryonic fibroblast) whole cell lysate. PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate. ICC/IF: HeLa cells, NIH/3T3 cells. Flow Cyt (intra): HeLa cells, NIH/3T3 cells. IP: HeLa and NIH/3T3 whole cell lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR26183-69

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290734の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP
1/30.
Flow Cyt (Intra)
1/500.
ICC/IF
1/50.
特記事項
WB
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP
1/30.
Flow Cyt (Intra)
1/500.
ICC/IF
1/50.
追加情報
Is unsuitable for IHC-P.

画像

  • Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
    Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
    All lanes : Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate.
    Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate.
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate.
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate.

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa


    Exposure time: 70 seconds


    Blocking / Diluent buffer and concentration:5% NFDM/TBST.

    Lysates should be made freshly and used in WB immediately to minimize protein degradation.

  • Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
    Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
    All lanes : Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734) at 1/1000 dilution

    Lane 1 : His-tagged human YTHDF1 recombinant protein 10ng
    Lane 2 : His-tagged human YTHDF2 recombinant protein 10ng
    Lane 3 : His-tagged human YTHDF3 recombinant protein 10ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa


    Exposure time: 70 seconds


    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
    Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized NIH/3T3 (mouse embryonic fibroblast) cells lebelling YTHDF1 + YTHDF2 + YTHDF3 with ab290734 at 1/50 (10.04 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/mL) dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/mL) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
    Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized HeLa (human cervix adenocarcinoma epithelial cell) cells lebelling YTHDF1 + YTHDF2 + YTHDF3 with ab290734 at 1/50 (10.04 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/mL) dilution (Green). Confocal image showing mostly cytoplasmic staining in Hela cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/mL) dilution.

  • Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
    Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling YTHDF1 + YTHDF2 + YTHDF3 with ab290734 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
    Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling YTHDF1 + YTHDF2 + YTHDF3 with ab290734 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
    Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)

    YTHDF1 + YTHDF2 + YTHDF3 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with ab290734 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab290734 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug

    Lane 2: ab290734 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab290734 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

     

  • Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)
    Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (ab290734)

    YTHDF1 + YTHDF2 + YTHDF3 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug ab290734 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using anti YTHDF1 + YTHDF2 + YTHDF3 antibody (ab290734) at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

    Lane 2: anti YTHDF1 + YTHDF2 + YTHDF3 antibody (ab290734) IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab290734 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

     

  • Anti-YTHDF1 antibody [EPR26183-69] (ab290734)
    Anti-YTHDF1 antibody [EPR26183-69] (ab290734)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab290734 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab290734 は 1 報の論文で使用されています。

  • Lin W  et al. Tumor-intrinsic YTHDF1 drives immune evasion and resistance to immune checkpoint inhibitors via promoting MHC-I degradation. Nat Commun 14:265 (2023). PubMed: 36650153

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