Anti-YTHDC2 抗体 (ab176846)
Key features and details
- Rabbit polyclonal to YTHDC2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-YTHDC2 antibody
YTHDC2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to YTHDC2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide within Human YTHDC2 aa 1200-1250. The exact sequence is proprietary. NP_073739.3
Sequence:K SSADTEFSDE CTTAERVLMK SPSPALHPPQ KYKDRGILHP KRGTEDRSDQ
Database link: Q9H6S0 -
ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab176846 was affinity purified using an epitope specific to YTHDC2 immobilized on solid support -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176846の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 160 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 160 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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配列類似性
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 2 ANK repeats.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 R3H domain.
Contains 1 YTH domain. - Information by UniProt
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参照データベース
- Entrez Gene: 462004 Chimpanzee
- Entrez Gene: 64848 Human
- Entrez Gene: 100173406 Orangutan
- Entrez Gene: 694612 Rhesus monkey
- SwissProt: Q9H6S0 Human
- Unigene: 231942 Human
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別名
- Probable ATP-dependent RNA helicase YTHDC2 antibody
- YTDC2_HUMAN antibody
- YTH domain containing 2 antibody
- Ythdc2 antibody
画像
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Western blot - Anti-YTHDC2 antibody (ab176846)All lanes : Anti-YTHDC2 antibody (ab176846) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 160 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-YTHDC2 antibody (ab176846)Detection of YTHDC2 by Western Blot of Immunoprecipitate.
ab176846 at 0.4 µg/ml labeling YTHDC2 in HeLa whole cell lysate immunoprecipitated using ab176846 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (10)
ab176846 は 10 報の論文で使用されています。
- Wang YJ et al. Reprogramming of m6A epitranscriptome is crucial for shaping of transcriptome and proteome in response to hypoxia. RNA Biol 18:131-143 (2021). PubMed: 32746693
- Ma L et al. Targeting SLC3A2 subunit of system XC- is essential for m6A reader YTHDC2 to be an endogenous ferroptosis inducer in lung adenocarcinoma. Free Radic Biol Med 168:25-43 (2021). PubMed: 33785413
- Yang X et al. METTL14 suppresses proliferation and metastasis of colorectal cancer by down-regulating oncogenic long non-coding RNA XIST. Mol Cancer 19:46 (2020). PubMed: 32111213
- Ma L et al. The m6A reader YTHDC2 inhibits lung adenocarcinoma tumorigenesis by suppressing SLC7A11-dependent antioxidant function. Redox Biol 38:101801 (2020). PubMed: 33232910
- Mao Y et al. m6A in mRNA coding regions promotes translation via the RNA helicase-containing YTHDC2. Nat Commun 10:5332 (2019). PubMed: 31767846
- Shi H et al. m6A facilitates hippocampus-dependent learning and memory through YTHDF1. Nature 563:249-253 (2018). PubMed: 30401835
- Murat P et al. RNA G-quadruplexes at upstream open reading frames cause DHX36- and DHX9-dependent translation of human mRNAs. Genome Biol 19:229 (2018). PubMed: 30591072
- Tan B et al. Viral and cellular N6-methyladenosine and N6,2'-O-dimethyladenosine epitranscriptomes in the KSHV life cycle. Nat Microbiol 3:108-120 (2018). PubMed: 29109479
- Muller M & Glaunsinger BA Nuclease escape elements protect messenger RNA against cleavage by multiple viral endonucleases. PLoS Pathog 13:e1006593 (2017). WB . PubMed: 28841715
- Wojtas MN et al. Regulation of m6A Transcripts by the 3'?5' RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline. Mol Cell 68:374-387.e12 (2017). PubMed: 29033321
