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AB109744

Anti-Yes1 抗体

Anti-Yes1 antibody

  • JP Deleterious:医薬用外劇物

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(3 Publications)

Rabbit Polyclonal Yes1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human YES1.

別名を表示する

YES, YES1, Tyrosine-protein kinase Yes, Proto-oncogene c-Yes, p61-Yes

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Yes1 antibody (AB109744)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Yes1 antibody (AB109744)

ab109744, at 10 µg/ml, staining Yes1 in formalin fixed, paraffin embedded Human placenta tissue by Immunohistochemistry.

Western blot - Anti-Yes1 antibody (AB109744)
  • WB

Unknown

Western blot - Anti-Yes1 antibody (AB109744)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Yes1 antibody (ab109744) at 1/1000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg

Predicted band size: 60 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Yes1 antibody (AB109744)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Yes1 antibody (AB109744)

Immunofluorescence analysis of Yes1 in methanol fixed HeLa cell, using ab109744 at a 1/200 dilution.
The upper image was stained with Hoesch 33342

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Yes1 antibody (AB109744)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Yes1 antibody (AB109744)

ab109744, at 10 μg/ml, staining Yes1 in formalin fixed, paraffin embedded Human breast tissue by Immunohistochemistry.

Western blot - Anti-Yes1 antibody (AB109744)
  • WB

Unknown

Western blot - Anti-Yes1 antibody (AB109744)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Yes1 antibody (ab109744) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 60 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

ICC/IF, IHC-P, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human YES1.

P07947

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Yes1 protein also known as p61-Yes is a member of the Src family kinases (SFKs) and has a molecular mass of approximately 61 kDa. This non-receptor tyrosine kinase engages in phosphorylation of tyrosine residues playing a significant role in cell signaling. It is widely expressed in many tissues including the brain heart and lungs suggesting its involvement in varied physiological processes.
Biological function summary

The Yes1 protein participates in cellular processes such as cell growth survival and differentiation. It contributes to signaling cascades that regulate these functions often working as part of protein complexes. Its ability to interact with various signaling molecules positions it as an important player in the control of cell behavior particularly in response to extracellular stimuli.

Pathways

The Yes1 protein integrates into key signaling routes like the epidermal growth factor receptor (EGFR) signaling pathway and the integrin signaling pathway. Within these pathways Yes1 interacts with proteins like c-Src and Fyn which are other members of the Src family. These pathways are vital in mediating cellular communication for growth and attachment highlighting Yes1's role in transmitting signals that prompt cellular responses.

Research connects Yes1 protein to several types of cancer such as colon cancer and breast cancer. The dysregulation of Yes1 along with associated proteins like c-Myc may lead to uncontrolled cell proliferation typical of tumorigenesis. Understanding these interactions offers insights into potential therapeutic targets as altering Yes1 activity might influence cancer progression or treatment outcomes.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity (PubMed : 26979622).
See full target information YES1
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Gut and liver 16:861-874 PubMed35686503

2022

Circ_0081143 Contributes to Gastric Cancer Malignant Development and Doxorubicin Resistance by Elevating the Expression of YES1 by Targeting mziR-129-2-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Ou,Lin Lin,Rihong Chen,Qingwen Xu,Caijin Zhou

Journal of clinical laboratory analysis 35:e24078 PubMed34708891

2021

miR-125a-5p promotes gastric cancer growth and invasion by regulating the Hippo pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruixin Li,Zhihao Hu,Zhuoyin Wang,Tianyu Zhu,Guojun Wang,Bulang Gao,Jingtao Wang,Xiumei Deng

Cancer management and research 13:3429-3442 PubMed33907466

2021

Matrine Regulates Proliferation, Apoptosis, Cell Cycle, Migration, and Invasion of Non-Small Cell Lung Cancer Cells Through the circFUT8/miR-944/YES1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hailing Zhu,Quan Lu,Qing Lu,Xuemin Shen,Liuyang Yu
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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