AB56701

Anti-YAP1 抗体 [2F12]

Name: Anti-YAP1 抗体 [2F12] (ab56701) | Abcam
Brand: Abcam Limited
SKU: AB56701
Price: 82000 JPY
Availability: InStock
Rating: 5 (5 reviews)

Anti-YAP1 antibody [2F12]

(5 Reviews)

(74 Publications)

Anti-YAP1 antibody [2F12] (ab56701) is a mouse monoclonal antibody detecting YAP1 in Western Blot, IHC-P. Suitable for Human, Mouse.

- Over 50 publications
- Trusted since 2007

別名を表示する

YAP65, YAP1, Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog

2 Images

Unknown

Western blot - Anti-YAP1 antibody [2F12] (AB56701)

YAP1 antibody (ab56701) at 1ug/lane + HeLa cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-YAP1 antibody [2F12] (ab56701)

Predicted band size: 54 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 antibody [2F12] (AB56701)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 antibody [2F12] (AB56701)

YAP1 antibody (ab56701) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium.

This image was generated using the ascites version of the product.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

2F12

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

キャリアフリー

Yes

交差種

Human, Mouse

アプリケーション

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human YAP1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P46937

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-YAP1 antibody [2F12] (ab56701) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of YAP1?
Anti-YAP1 [2F12] (ab56701) specifically detects a band for YAP1 (UniProt: P46937) at a molecular weight of 49kDa.

Trusted by the scientific community
Anti-YAP1 [2F12] (ab56701) was first used in a scientific publication in 2007 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-YAP1 [2F12] (ab56701) has over 5 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

pH: 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

YAP1 also known as Yes-associated protein 1 is a transcriptional coactivator involved in regulating gene expression. This protein has a mass of approximately 65 kDa and is expressed in various tissues including the liver and heart. YAP1 plays a significant role in the Hippo signaling pathway where it acts by binding to transcriptional enhancer factors such as TEADs to activate gene transcription.

Biological function summary

YAP1 influences cell proliferation and apoptosis by interacting with other proteins to form transcriptional complexes. It participates in promoting organ size and tissue regeneration. YAP1's activity is regulated through phosphorylation primarily at serine 127 which determines its subcellular localization and transcriptional activity. The phosphorylated form often referred to as phospho-YAP remains in the cytoplasm while dephosphorylated YAP1 translocates to the nucleus where it contributes to transcriptional activation.

Pathways

YAP1 is importantly involved in the Hippo signaling pathway and Wnt/Β-catenin signaling pathway. In the Hippo pathway kinases LATS1/2 phosphorylate YAP1 preventing its nuclear localization and promoting cell apoptosis when required. In contrast in the Wnt/Β-catenin pathway YAP1 interacts with Β-catenin to regulate gene expression related to proliferation. Proteins such as TAZ which shares structural and functional similarities with YAP1 often collaborate in these pathways influencing cellular growth and differentiation.

YAP1's dysregulation links to cancer development notably in liver and breast cancer. Overexpression or nuclear accumulation of YAP1 leads to uncontrollable cell growth and tumorigenesis. Its interaction with the TEAD transcription factors highlights its significance in oncogenesis. Furthermore YAP1 also connects with pathways involving other proteins like TGF-Β contributing to fibrosis-related disorders such as pulmonary fibrosis where unexpected remodeling and excessive tissue growth occur.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The protein expressed by the YAP1 gene functions as a transcriptional regulator, acting both as a coactivator and corepressor. It is a critical downstream regulatory target in the Hippo signaling pathway, which is crucial for organ size control and tumor suppression, as it restricts proliferation and promotes apoptosis. The pathway involves a kinase cascade where STK3/MST2 and STK4/MST1 activate LATS1/2, which then inactivates the YAP1 oncoprotein. YAP1 plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation through ARHGAP18. It controls cell proliferation in response to cell contact, with its phosphorylation by LATS1/2 preventing nuclear translocation necessary for regulating cellular genes linked to proliferation, death, and migration. TEAD transcription factors are needed for YAP1 to stimulate gene expression, cell growth, anchorage-independent growth, and EMT induction. Additionally, YAP1 suppresses ciliogenesis by repressing TEAD4 target genes AURKA and PLK1. Isoforms 2 and 3 of YAP1 activate the C-terminal fragment of ERBB4 (isoform 3). This supplementary information is collated from multiple sources and compiled automatically.

See full target information YAP1

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文献 (74)

Recent publications for all applications. Explore the full list and refine your search

Folia neuropathologica 63:127-137 PubMed40755337

2025

Effect of the TGF-b signaling pathway on spinal cord ependymoma: A study based on bioinformatics analysis and clinical trials.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Dong Fan,Man-Li Zhu,Mamutijiang Muertizha,Jia-Ming Wang,Kun Luo

Theranostics 15:1930-1948 PubMed39897564

2025

YAP1 mediates the dimensional and chemical coordination of immunoregulation and therapy in extensively passaged mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Ying Du,Feng Zhou,Na Zhao,Lei Bao,Cheng-Biao Hu,Jing Lei,An-Qi Liu,Ying-Feng Gao,Li-Hui Bao,Hua Ni,Xiao-Rui Yu,Ji Chen,Bing-Dong Sui

Cell proliferation 58:e13794 PubMed39710429

2024

The Dual Roles of Lamin A/C in Macrophage Mechanotransduction.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Wang,Sabine Ruf,Lei Wang,Thomas Heimerl,Gert Bange,Sabine Groeger

Cancer research 84:4283-4297 PubMed39356622

2024

Induction of the TEAD Coactivator VGLL1 by Estrogen Receptor-Targeted Therapy Drives Resistance in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Gemma,Chun-Fui Lai,Anup K Singh,Antonino Belfiore,Neil Portman,Heloisa Z Milioli,Manikandan Periyasamy,Sara Raafat,Alyssa J Nicholls,Claire M Davies,Naina R Patel,Georgia M Simmons,Hailing Fan,Van T M Nguyen,Luca Magnani,Emad Rakha,Lesley-Ann Martin,Elgene Lim,R Charles Coombes,Giancarlo Pruneri,Laki Buluwela,Simak Ali

Science advances 10:eadl6366 PubMed39028807

2024

A fibronectin gradient remodels mixed-phase mesoderm.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zhu,Bin Gu,Evan C Thomas,Yunyun Huang,Yun-Kyo Kim,Hirotaka Tao,Theodora M Yung,Xin Chen,Kaiwen Zhang,Elizabeth K Woolaver,Mikaela R Nevin,Xi Huang,Rudolph Winklbauer,Janet Rossant,Yu Sun,Sevan Hopyan

Materials today. Bio 26:101104 PubMed38952539

2024

Turning sublimed sulfur and bFGF into a nanocomposite to accelerate wound healing via co-activate FGFR and Hippo signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jieqiong Cao,Zijian Su,Yibo Zhang,Zhiqi Chen,Jingsheng Li,Yulin Cai,Yiming Chang,Minghua Lei,Qianyi He,Weicai Li,Xuan Liao,Shuixing Zhang,An Hong,Xiaojia Chen

Histology and histopathology 40:57-65 PubMed38785315

2024

Association of low angiomotin-p130 and high YAP1 nuclear expression with adverse prognosis in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junna Cai,Xiaorui Han,Meng Li,Xiaoli Liu,Fengying Zhang,Xiaohua Wu

Biophysical journal 123:1222-1239 PubMed38605521

2024

Nuclear curvature determines Yes-associated protein localization and differentiation of mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ajinkya Ghagre,Alice Delarue,Luv Kishore Srivastava,Newsha Koushki,Allen Ehrlicher

EBioMedicine 102:105062 PubMed38492534

2024

Comprehensive analysis of transcription factor-based molecular subtypes and their correlation to clinical outcomes in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sehhoon Park,Tae Hee Hong,Soohyun Hwang,Simon Heeke,Carl M Gay,Jiyeon Kim,Hyun-Ae Jung,Jong-Mu Sun,Jin Seok Ahn,Myung-Ju Ahn,Jong Ho Cho,Yong Soo Choi,Jhingook Kim,Young Mog Shim,Hong Kwan Kim,Lauren Averett Byers,John V Heymach,Yoon-La Choi,Se-Hoon Lee,Keunchil Park

The Journal of cell biology 223: PubMed38477830

2024

N-cadherin dynamically regulates pediatric glioma cell migration in complex environments.

Applications

Unspecified application

Species

Unspecified reactive species

Dayoung Kim,James M Olson,Jonathan A Cooper

View all publications

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