Anti-YAF2 抗体 [EPR11020] (ab177945)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11020] to YAF2
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-YAF2 antibody [EPR11020]
YAF2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11020] to YAF2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human skeletal muscle and fetal heart lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11020 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177945の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
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IP |
1/10 - 1/100.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
IP
1/10 - 1/100. |
ターゲット情報
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機能
Binds to MYC and inhibits MYC-mediated transactivation. Also binds to MYCN and enhances MYCN-dependent transcriptional activation. Increases calpain 2-mediated proteolysis of YY1 in vitro. Component of the E2F6.com-1 complex, a repressive complex that methylates 'Lys-9' of histone H3, suggesting that it is involved in chromatin-remodeling. -
配列類似性
Contains 1 RanBP2-type zinc finger. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10138 Human
- Entrez Gene: 67057 Mouse
- Entrez Gene: 690262 Rat
- Omim: 607534 Human
- SwissProt: Q8IY57 Human
- SwissProt: Q99LW6 Mouse
- Unigene: 649195 Human
- Unigene: 708084 Human
see all -
別名
- MGC41856 antibody
- YAF2 antibody
- YAF2_HUMAN antibody
- YY1-associated factor 2 antibody
画像
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All lanes : Anti-YAF2 antibody [EPR11020] (ab177945) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Fetal heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 20 kDa -
Western blot analysis on immunoprecipitation pellet from (1) Human skeletal muscle lysate or (2) 1X PBS (negative control) using ab177945 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab177945 は 1 報の論文で使用されています。
- Yue S et al. Cryptotanshinone interferes with chondrocyte apoptosis in osteoarthritis by inhibiting the expression of miR-574-5p. Mol Med Rep 23:N/A (2021). PubMed: 33878859