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AB321987

Anti-XRCC3 抗体 [EPR28957-35] - BSA and Azide free

Anti-XRCC3 antibody [EPR28957-35] - BSA and Azide free

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Rabbit Recombinant Monoclonal XRCC3 antibody. Carrier free. Suitable for WB and reacts with Human samples.

別名を表示する

DNA repair protein XRCC3, X-ray repair cross-complementing protein 3, XRCC3

2 Images
Western blot - Anti-XRCC3 antibody [EPR28957-35] - BSA and Azide free (AB321987)
  • WB

Supplier Data

Western blot - Anti-XRCC3 antibody [EPR28957-35] - BSA and Azide free (AB321987)

This data was developed using ab321986, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : kidney, liver(PMID : 9660962)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-XRCC3 antibody [EPR28957-35] (<a href='/products/primary-antibodies/xrcc3-antibody-epr28957-35-ab321986'>ab321986</a>) at 1/1000 dilution

Lane 1:

SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

DU 145 (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

Human testis tissue lysate at 40 µg

Lane 4:

Human liver tissue lysate at 40 µg

Lane 5:

Human kidney tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 38 kDa,36 kDa

false

Exposure time: 158s

Western blot - Anti-XRCC3 antibody [EPR28957-35] - BSA and Azide free (AB321987)
  • WB

Supplier Data

Western blot - Anti-XRCC3 antibody [EPR28957-35] - BSA and Azide free (AB321987)

This data was developed using ab321986, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-XRCC3 antibody [EPR28957-35] (<a href='/products/primary-antibodies/xrcc3-antibody-epr28957-35-ab321986'>ab321986</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

Lane 2:

HeLa transfected with siRNA specifically targeting XRCC3 whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 38 kDa,36 kDa

false

Exposure time: 180s

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR28957-35

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

ab321987 is the carrier-free version of ab321986.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

XRCC3 also known as X-Ray Repair Cross Complementing 3 plays an important role in DNA repair processes. It is a protein weighing approximately 39 kDa and is expressed in many tissues within the human body. This protein belongs to the RAD51 family of recombinases and is essential during homologous recombination ensuring the stability and integrity of the genome by assisting in the repair of double-strand breaks in DNA.
Biological function summary

XRCC3 promotes DNA repair and genomic stability and functions as part of a complex. It associates with other proteins in the RAD51 family to facilitate the proper repair mechanisms required for maintaining cellular health. XRCC3’s activity in homologous recombination is critical to prevent DNA damage accumulation which could lead to chromosomal abnormalities impacting cell survival and proliferation.

Pathways

XRCC3 is integrally involved in homologous recombination repair and the broader DNA damage response network. It interacts closely with RAD51 and BRCA2 to mediate accurate DNA repair by homologous recombination ensuring precise repair pathway activation and reducing erroneous repair activities. XRCC3 supports the recruitment of RAD51 to DNA breaks and collaborates with the Fanconi anemia pathway to protect against genomic instability.

XRCC3 mutations or dysfunctions have associations with cancer susceptibility particularly breast cancer. The protein’s role in DNA repair links it to other proteins like BRCA1 and BRCA2 which have established connections in hereditary breast and ovarian cancer syndromes. Altered XRCC3 expression or sequence changes can compromise DNA repair pathways heightening the risk of tumorigenesis due to unchecked DNA damage accumulation.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD51 paralog protein complex CX3 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
See full target information DNA repair protein XRCC3
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