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リコンビナントRabMAb

Anti-XPG 抗体 [EPR25351-5] - BSA and Azide free (ab302616)

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Western blot - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
  • Western blot - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
  • Western blot - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
  • Immunocytochemistry/ Immunofluorescence - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
  • Flow Cytometry (Intracellular) - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
  • Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25351-5] to XPG - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-XPG antibody [EPR25351-5] - BSA and Azide free
    XPG 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25351-5] to XPG - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt (Intra), WBmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, Daudi, HaCaT, SK-OV-3, whole cell lysates; HeLa transfected with siRNA specifically targeting XPG and scrambled siRNA control whole cell lysates; human normal thymus, and ovary cancer tissue lysates, ICC/IF:HeLa cells. Flow Cyt (Intra): HeLa cells.

  • 特記事項

    ab302616 is the carrier-free version of ab302615.

     

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302616の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
特記事項
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too.

  • 関連疾患

    Defects in ERCC5 are the cause of xeroderma pigmentosum complementation group G (XP-G) [MIM:278780]; also known as xeroderma pigmentosum VII (XP7). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-G patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.

  • 配列類似性

    Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • COFS 3 antibody
      • COFS3 antibody
      • DNA excision repair protein ERCC 5 antibody
      • DNA excision repair protein ERCC-5 antibody
      • DNA excision repair protein ERCC5 antibody
      • DNA repair protein complementing XP G cells antibody
      • DNA repair protein complementing XP-G cells antibody
      • DNA repair protein complementing XPG cells antibody
      • ERCC 5 antibody
      • ERCC5 antibody
      • ERCC5_HUMAN antibody
      • ERCM 2 antibody
      • ERCM2 antibody
      • Excision repair cross complementation group 5 antibody
      • Excision Repair Cross Complementing Rodent Repair Deficiency antibody
      • Excision repair cross complementing rodent repair deficiency complementation group 5 antibody
      • Excision repair protein antibody
      • OTTHUMP00000064902 antibody
      • UVDR antibody
      • Xeroderma Pigmentosum Complementation Group G antibody
      • Xeroderma pigmentosum complementation group G protein antibody
      • Xeroderma pigmentosum group G complementing protein antibody
      • Xeroderma pigmentosum group G-complementing protein antibody
      • XPG antibody
      • XPG complementing protein antibody
      • XPGC antibody
      see all

    画像

    • Western blot - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
      Western blot - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
      All lanes : Anti-XPG antibody [EPR25351-5] (ab302615) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
      Lane 2 : HeLa transfected with siRNA specifically targeting XPG, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 133 kDa
      Observed band size: 133 kDa


      Exposure time: 26 seconds


      This data was developed using ab302615, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

      Samples are non-boiled as boiling may cause protein aggregates.

    • Western blot - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
      Western blot - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
      All lanes : Anti-XPG antibody [EPR25351-5] (ab302615) at 1/1000 dilution

      Lane 1 : Human thymus tissue lysate
      Lane 2 : Human ovary cancer tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

      Predicted band size: 133 kDa
      Observed band size: 133 kDa



      This data was developed using ab302615, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

      Exposure times:

      Lane 1: 103 seconds

      Lane 2: 180 seconds.

      Samples are non-boiled as boiling may cause protein aggregates.

    • Western blot - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
      Western blot - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
      All lanes : Anti-XPG antibody [EPR25351-5] (ab302615) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate
      Lane 3 : HaCaT (human skin keratinocyte) whole cell lysate
      Lane 4 : SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 133 kDa
      Observed band size: 133 kDa


      Exposure time: 180 seconds


      This data was developed using ab302615, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

      Samples are non-boiled as boiling may cause protein aggregates.

    • Immunocytochemistry/ Immunofluorescence - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
      Immunocytochemistry/ Immunofluorescence - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)

      This data was developed using ab302615, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling XPG with ab302615 at 1/100 (5.03 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing nuclear staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

    • Flow Cytometry (Intracellular) - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
      Flow Cytometry (Intracellular) - Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)

      This data was developed using ab302615, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling XPG with ab302615 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

    • Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)
      Anti-XPG antibody [EPR25351-5] - BSA and Azide free (AB302616)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302616 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302616 は論文での使用が確認できていません。

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