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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-XPF 抗体 [EPR25350-148] - BSA and Azide free (ab290740)

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Western blot - Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)
  • Western blot - Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)
  • Immunoprecipitation - Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)
  • Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25350-148] to XPF - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-XPF antibody [EPR25350-148] - BSA and Azide free
    XPF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25350-148] to XPF - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate, K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate, Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate, 293T (human embryonic kidney epithelial cell) whole cell lysate. IP: 293T (human embryonic kidney epithelial cell) whole cell lysate.

  • 特記事項

    ab290740 is a carrier free version of ab290725. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290740の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 104 kDa.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 104 kDa.
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.

  • 関連疾患

    Defects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F) [MIM:278760]; also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities.
    Defects in ERCC4 are a cause of XFE progeroid syndrome (XFEPS) [MIM:610965]. This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly.

  • 配列類似性

    Belongs to the XPF family.
    Contains 1 ERCC4 domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DNA excision repair protein ERCC 4 antibody
      • DNA excision repair protein ERCC-4 antibody
      • DNA excision repair protein ERCC4 antibody
      • DNA repair endonuclease XPF antibody
      • DNA repair protein complementing XP F cells antibody
      • DNA repair protein complementing XP-F cells antibody
      • ERCC 11 antibody
      • ERCC 4 antibody
      • ERCC11 antibody
      • ERCC4 antibody
      • Excision repair complementing defective in Chinese hamster antibody
      • Excision repair cross complementing rodent repair deficiency complementation group 4 antibody
      • excision repair cross-complementation group 4 antibody
      • FANCQ antibody
      • RAD 1 antibody
      • RAD1 antibody
      • Xeroderma pigmentosum complementation group F antibody
      • Xeroderma pigmentosum group F complementing protein antibody
      • Xeroderma pigmentosum group F-complementing protein antibody
      • Xeroderma pigmentosum VI antibody
      • XP, group G antibody
      • XP6 antibody
      • XPF_HUMAN antibody
      see all

    画像

    • Western blot - Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)
      Western blot - Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)
      All lanes : Anti-XPF antibody [EPR25350-148] (ab290725) at 1/1000 dilution

      Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate.
      Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate.
      Lane 3 : 293T (human embryonic kidney epithelial cell) whole cell lysate.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 104 kDa
      Observed band size: 104 kDa


      Exposure time: 15 seconds


      This data was developed using ab290725, the same antibody clone in a different buffer formulation. 

      Blocking / Dilution buffer and concentration: 5% NFDM/TBST

      Lysates should be made freshly and used in WB immediately to minimize protein degradation.

    • Western blot - Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)
      Western blot - Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)
      All lanes : Anti-XPF antibody [EPR25350-148] (ab290725) at 1/1000 dilution

      Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate.
      Lane 2 : ERCC4 knockout HeLa whole cell lysate.
      Lane 3 : 293T (human embryonic kidney epithelial cell) whole cell lysate.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution

      Predicted band size: 104 kDa
      Observed band size: 104 kDa



      This data was developed using ab290725, the same antibody clone in a different buffer formulation. 

      Blocking / Diluent buffer and concentration:Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

      Lanes 1-3: Merged signal (red and green). Green - ab290725 observed at 104 kDa. Red - loading control ab8245 observed at 36 kDa.

      ab290725 Anti-ERCC4 antibody [EPR25350-148] was shown to specifically react with ERCC4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line was used. Wild-type and ERCC4 knockout samples were subjected to SDS-PAGE. ab290725 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    • Immunoprecipitation - Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)
      Immunoprecipitation - Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)

      This data was developed using ab290725, the same antibody clone in a different buffer formulation.

      XPF was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug with ab290725 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab290725 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug

      Lane 2: ab290725 IP in 293T whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab290725 in 293T whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 15 seconds

    • Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)
      Anti-XPF antibody [EPR25350-148] - BSA and Azide free (ab290740)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290740 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290740 は論文での使用が確認できていません。

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