Anti-XPF 抗体 (ab76948)
Key features and details
- Rabbit polyclonal to XPF
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-XPF antibody
XPF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to XPF -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide corresponding to a region between residues 855 and 905 of human XPF (AAB07689.1)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab76948 was affinity purified using an epitope specific to XPF immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76948の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 103 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 103 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link. -
関連疾患
Defects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F) [MIM:278760]; also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities.
Defects in ERCC4 are a cause of XFE progeroid syndrome (XFEPS) [MIM:610965]. This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly. -
配列類似性
Belongs to the XPF family.
Contains 1 ERCC4 domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2072 Human
- Omim: 278760 Human
- SwissProt: Q92889 Human
- Unigene: 567265 Human
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別名
- DNA excision repair protein ERCC 4 antibody
- DNA excision repair protein ERCC-4 antibody
- DNA excision repair protein ERCC4 antibody
see all
画像
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Western blot - Anti-XPF antibody (ab76948)All lanes : Anti-XPF antibody (ab76948) at 1/2000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ercc4 knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-XPF antibody staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab76948 was shown to bind specifically to XPF. A band was observed at 105 kDa in wild-type HeLa cell lysates with no signal observed at this size in ercc4 knockout cell line ab280068 (knockout cell lysate ab280127). To generate this image, wild-type and ercc4 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Western blot - Anti-XPF antibody (ab76948)All lanes : Anti-XPF antibody (ab76948) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 103 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPF antibody (ab76948)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling XPF with ab76948 at 1/200 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-XPF antibody (ab76948)ab76948 at 1µg/ml staining XPF in HeLa whole cell lysate immunoprecipitated using ab76948 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab76948 は 7 報の論文で使用されています。
- Chen J et al. Exploiting the acquired vulnerability of cisplatin-resistant tumors with a hypoxia-amplifying DNA repair-inhibiting (HYDRI) nanomedicine. Sci Adv 7:N/A (2021). PubMed: 33771859
- Dashner-Titus EJ et al. Differential response of human T-lymphocytes to arsenic and uranium. Toxicol Lett 333:269-278 (2020). PubMed: 32866568
- Edwards DS et al. BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation. Cell Rep 32:108166 (2020). PubMed: 32966794
- Li M et al. MicroRNA-200c reverses drug resistance of human gastric cancer cells by targeting regulation of the NER-ERCC3/4 pathway. Oncol Lett 18:145-152 (2019). PubMed: 31289483
- Cristini A et al. Dual Processing of R-Loops and Topoisomerase I Induces Transcription-Dependent DNA Double-Strand Breaks. Cell Rep 28:3167-3181.e6 (2019). PubMed: 31533039
- Popp I et al. Fanconi anemia with sun-sensitivity caused by a Xeroderma pigmentosum-associated missense mutation in XPF. BMC Med Genet 19:7 (2018). PubMed: 29325523
- Cavallo F et al. Reduced proficiency in homologous recombination underlies the high sensitivity of embryonal carcinoma testicular germ cell tumors to Cisplatin and poly (adp-ribose) polymerase inhibition. PLoS One 7:e51563 (2012). WB ; Mouse . PubMed: 23251575