Anti-XIN (phospho S295) 抗体 (ab187632)
Key features and details
- Rabbit polyclonal to XIN (phospho S295)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-XIN (phospho S295) antibody
XIN 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to XIN (phospho S295) -
由来種
Rabbit -
特異性
Specific for the ~250k and ~150k splice variants of XIN phosphorylated at Ser295. Immunolabeling of the XIN band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein).
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Dog, Human, Non human primates
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免疫原
Synthetic peptide within Mouse XIN (phospho S295). The exact sequence is proprietary. Phosphopeptide corresponding to amino acid residues surrounding the phospho-Serine at 295.
Database link: O70373 -
ポジティブ･コントロール
- Mouse heart lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.24% HEPES, 0.87% Sodium chloride, 0.01% BSA, 50% Glycerol -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187632の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 123 kDa.
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| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 123 kDa. |
ターゲット情報
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機能
Protects actin filaments from depolymerization. -
組織特異性
Isoform A, isoform B and isoform C are expressed in heart. -
配列類似性
Belongs to the Xin family.
Contains 17 Xin repeats. -
ドメイン
Xin repeats bind F-actin. -
細胞内局在
Cell junction. Colocalizes with actin stress fibers. - Information by UniProt
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参照データベース
- Entrez Gene: 165904 Human
- Entrez Gene: 22437 Mouse
- Entrez Gene: 316071 Rat
- Omim: 609777 Human
- SwissProt: Q702N8 Human
- SwissProt: O70373 Mouse
- Unigene: 447868 Human
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別名
- AI415219 antibody
- Cardiomyopathy-associated protein 1 antibody
- CMYA1 antibody
see all
画像
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Western blot - Anti-XIN (phospho S295) antibody (ab187632)All lanes : Anti-XIN (phospho S295) antibody (ab187632) at 1/1000 dilution
Lane 1 : Mouse heart lysate with untreated
Lane 2 : Mouse heart lysate with treated with Lambda phosphatase
Predicted band size: 123 kDaBoth the ~250k and ~150k splice variants of the XIN protein phosphorylated at Serine
295 are labeled.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab187632 は 1 報の論文で使用されています。
- Williams AL et al. miR-125 family regulates XIRP1 and FIH in response to myocardial infarction. Physiol Genomics 52:358-368 (2020). PubMed: 32716698