Anti-XIAP 抗体 (ab2541)
Key features and details
- Rabbit polyclonal to XIAP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-XIAP antibody
XIAP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to XIAP -
由来種
Rabbit -
特異性
This antibody detects the 53 kDa human XIAP protein. It does not cross-react with other apoptosis inhibitors. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to XIAP. Available on request. (Peptide available as ab38275.)
-
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 3183 XIAP Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2541の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
ターゲット情報
-
機能
Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. -
組織特異性
Ubiquitous, except peripheral blood leukocytes. -
関連疾患
Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma. -
配列類似性
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger. -
ドメイン
The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains. -
翻訳後修飾
Ubiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 331 Human
- Omim: 300079 Human
- SwissProt: P98170 Human
- Unigene: 356076 Human
-
別名
- AP 13 antibody
- API3 antibody
- Apoptosis Inhibitor 3 antibody
see all
画像
-
Western blot - Anti-XIAP antibody (ab2541)All lanes : Anti-XIAP antibody (ab2541) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 12 minutes
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (12)
ab2541 は 12 報の論文で使用されています。
- Mendonça BS et al. Subcellular localization of X-linked inhibitor of apoptosis protein (XIAP) in cancer: Does that matter? BBA Adv 2:100050 (2022). PubMed: 37082602
- Zhou S et al. Gambogenic acid suppresses bladder cancer cells growth and metastasis by regulating NF-κB signaling. Chem Biol Drug Des 96:1272-1279 (2020). PubMed: 32491272
- Wang X Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide-induced acute pneumonia in rats. Mol Med Rep 22:3127-3134 (2020). PubMed: 32945404
- Deng W et al. MicroRNA-130a regulates neurological deficit and angiogenesis in rats with ischaemic stroke by targeting XIAP. J Cell Mol Med 24:10987-11000 (2020). PubMed: 32790238
- Dong X et al. Effect of Jiawei Fengshining on Synovial Cell Apoptosis and TGF-ß1/Smad Signaling Pathway in Rats with Rheumatoid Arthritis. Evid Based Complement Alternat Med 2019:8614034 (2019). PubMed: 31929822