Anti-XBP1 抗体 (ab37152)
Key features and details
- Rabbit polyclonal to XBP1
- Suitable for: IHC-P, IHC-Fr, ICC, IHC (PFA fixed), WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-XBP1 antibody
XBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to XBP1 -
由来種
Rabbit -
特異性
XBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1. -
アプリケーション
適用あり: IHC-P, IHC-Fr, ICC, IHC (PFA fixed), WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow
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免疫原
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ポジティブ･コントロール
- WB: HepG2 cell lysate, A549 cell lysate. IHC-P: Human liver and non-lesional skin tissue; Mouse testis and spleen tissue, rat liver tissue. ICC/IF: HepG2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab37152の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (12) |
Use a concentration of 2 - 5 µg/ml.
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| IHC-Fr |
Use at an assay dependent concentration. PubMed: 20028872
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| ICC |
Use a concentration of 10 µg/ml.
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| IHC (PFA fixed) |
Use a concentration of 20 µg/ml.
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| WB | (11) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910).
Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). |
| ICC/IF | (3) |
Use a concentration of 20 µg/ml.
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| 特記事項 |
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IHC-P
Use a concentration of 2 - 5 µg/ml. |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 20028872 |
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ICC
Use a concentration of 10 µg/ml. |
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IHC (PFA fixed)
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human XBP1 peptide (ab39910). Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). |
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ICC/IF
Use a concentration of 20 µg/ml. |
ターゲット情報
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機能
Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. -
関連疾患
Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. -
配列類似性
Belongs to the bZIP family.
Contains 1 bZIP domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7494 Human
- Entrez Gene: 22433 Mouse
- Entrez Gene: 289754 Rat
- Omim: 194355 Human
- SwissProt: P17861 Human
- SwissProt: O35426 Mouse
- SwissProt: Q9R1S4 Rat
- Unigene: 437638 Human
see all -
別名
- Tax responsive element binding protein 5 antibody
- Tax-responsive element-binding protein 5 antibody
- TREB5 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling XBP1 with ab37152 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
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Western blot - Anti-XBP1 antibody (ab37152)All lanes : Anti-XBP1 antibody (ab37152) at 1 µg/ml
Lane 1 : Rat Liver Tissue Lysate
Lane 2 : Rat Liver Tissue Lysate with Blocking peptide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa1h incubation at RT in 5% NFDM/TBST.
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Western blot - Anti-XBP1 antibody (ab37152)Anti-XBP1 antibody (ab37152) at 4 µg/ml + Mouse Pancreas Tissue lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa1h incubation at RT in 5% NFDM/TBST.
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Western blot - Anti-XBP1 antibody (ab37152)All lanes : Anti-XBP1 antibody (ab37152) at 4 µg/ml
Lane 1 : Wild Type HeLa cells
Lane 2 : XBP-1 knockout HeLa cells
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa1h incubation at RT in 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Immunohistochemical analysis of paraffin-embedded Mouse testis Tissue using ab37152 at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary antibody. Counter stained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Immunohistochemical analysis of paraffin-embedded Mouse spleen Tissue using ab37152 at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary antibody. Counter stained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Immunohistochemical analysis of paraffin-embedded Rat Liver Tissue using ab37152 at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Western blot - Anti-XBP1 antibody (ab37152)Lane 1 : Anti-XBP1 antibody (ab37152) at 0.25 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 0.5 µg/ml
Lane 3 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
All lanes : A549 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa1h incubation at RT in 5% NFDM/TBST.
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Western blot - Anti-XBP1 antibody (ab37152)Lane 1 : Anti-XBP1 antibody (ab37152) at 1 µg/ml
Lane 2 : Anti-XBP1 antibody (ab37152) at 2 µg/ml
All lanes : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa -
Immunohistochemistry (PFA fixed) - Anti-XBP1 antibody (ab37152)Immunohistochemistry of human liver tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).
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Immunohistochemistry (PFA fixed) - Anti-XBP1 antibody (ab37152)Immunohistochemistry of mouse pancreas tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).
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Immunohistochemistry (PFA fixed) - Anti-XBP1 antibody (ab37152)Immunohistochemistry of human pancreas tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Image courtesy of an anonymous Abreview.ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)Image from Ren Yunqing et.al., PLoS Genet. 2009 June; 5(6): e1000523 (Fig 6C).ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (165)
ab37152 は 165 報の論文で使用されています。
- Jia X et al. XBP1-elicited environment by chemotherapy potentiates repopulation of tongue cancer cells by enhancing miR-22/lncRNA/KAT6B-dependent NF-κB signalling. Clin Transl Med 13:e1166 (2023). PubMed: 36639835
- Deng J et al. Human-induced pluripotent stem cell-derived cerebral organoid of leukoencephalopathy with vanishing white matter. CNS Neurosci Ther 29:1049-1066 (2023). PubMed: 36650674
- Zhang B et al. MSX1 Regulates Goat Endometrial Function by Altering the Plasma Membrane Transformation of Endometrial Epithelium Cells during Early Pregnancy. Int J Mol Sci 24:N/A (2023). PubMed: 36835532
- Ji J et al. A novel variant in NBAS identified from an infant with fever-triggered recurrent acute liver failure disrupts the function of the gene. Hum Genome Var 10:13 (2023). PubMed: 37055399
- Wang J et al. PARP16-Mediated Stabilization of Amyloid Precursor Protein mRNA Exacerbates Alzheimer's Disease Pathogenesis. Aging Dis 14:1458-1471 (2023). PubMed: 37163422