Anti-Xanthine Oxidase 抗体 [EPR4605] (ab109235)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4605] to Xanthine Oxidase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Xanthine Oxidase antibody [EPR4605]
Xanthine Oxidase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4605] to Xanthine Oxidase -
由来種
Rabbit -
特異性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human fetal liver, Mouse and Rat liver tissue lysates, 293T cell lysates, Human milk IHC-P: Human liver tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4605 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-Xanthine Oxidase antibody [EPR4605] - BSA and Azide free (ab246338)
- PE Anti-Xanthine Oxidase antibody [EPR4605] (ab317895)
- APC Anti-Xanthine Oxidase antibody [EPR4605] (ab317933)
- Alexa Fluor® 488 Anti-Xanthine Oxidase antibody [EPR4605] (ab317973)
- Alexa Fluor® 594 Anti-Xanthine Oxidase antibody [EPR4605] (ab318014)
- Alexa Fluor® 555 Anti-Xanthine Oxidase antibody [EPR4605] (ab318056)
- Alexa Fluor® 647 Anti-Xanthine Oxidase antibody [EPR4605] (ab318098)
- Alexa Fluor® 750 Anti-Xanthine Oxidase antibody [EPR4605] (ab320939)
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109235の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 146 kDa).
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IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 146 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ターゲット情報
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機能
Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). -
組織特異性
Detected in milk (at protein level). -
関連疾患
Defects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. -
配列類似性
Belongs to the xanthine dehydrogenase family.
Contains 1 2Fe-2S ferredoxin-type domain.
Contains 1 FAD-binding PCMH-type domain. -
翻訳後修飾
Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). -
細胞内局在
Cytoplasm. Peroxisome. Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7498 Human
- Entrez Gene: 22436 Mouse
- Entrez Gene: 497811 Rat
- Omim: 607633 Human
- SwissProt: P47989 Human
- SwissProt: Q00519 Mouse
- SwissProt: P22985 Rat
- Unigene: 250 Human
see all -
別名
- Xanthine dehydrogenase antibody
- Xanthine dehydrogenase/oxidase antibody
- Xanthine oxidase antibody
see all
画像
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All lanes : Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/10000 dilution (Purified)
Lane 1 : Mouse liver lysates
Lane 2 : Rat liver lysates
Lane 3 : Mouse heart lysates
Lane 4 : Mouse brain lysates
Lane 5 : Mouse lung lysates
Lane 6 : Mouse kidney lysates
Lane 7 : Mouse spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 146 kDa
Observed band size: 146 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
Xanthine Oxidase is abundantly expressed in liver and low in heart, brain and lung as what is described in PMID: 10462034
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Xanthine Oxidase with purified ab109235 at 1/100 dilution (2.3 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/5000 dilution (Purified) + Human milk lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 146 kDa
Observed band size: 146 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal liver lysate
Lane 2 : 293T lysate
Lane 3 : Human milk lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 146 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)
Immunohistochemical analysis of Xanthine Oxidase expression in paraffin-embedded Human liver tissue using ab109235 (unpurified) at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab109235 は 29 報の論文で使用されています。
- Miller SG et al. Uric acid formation is driven by crosstalk between skeletal muscle and other cell types. JCI Insight 9:N/A (2024). PubMed: 38032735
- An MF et al. Anti-hyperuricemia effect of hesperetin is mediated by inhibiting the activity of xanthine oxidase and promoting excretion of uric acid. Front Pharmacol 14:1128699 (2023). PubMed: 37124197
- Ito A et al. Effects of the angiotensin-converting enzyme inhibitor captopril on occlusal-disharmony-induced cardiac dysfunction in mice. Sci Rep 13:19927 (2023). PubMed: 37968296
- Kiyomoto K et al. Oral angiotensin-converting enzyme inhibitor captopril protects the heart from Porphyromonas gingivalis LPS-induced cardiac dysfunction in mice. PLoS One 18:e0292624 (2023). PubMed: 37983238
- Hayakawa Y et al. Vidarabine, an anti-herpes agent, prevents occlusal-disharmony-induced cardiac dysfunction in mice. J Physiol Sci 72:2 (2022). PubMed: 35148678